Method for separating apoplast juice from plant stem tissues

A technology of plant stems and apoplasts, applied in the preparation of test samples, etc.

Inactive Publication Date: 2015-01-14
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the isolation of the extraplasmic fluid of plants with degenerated aerial parts.

Method used

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  • Method for separating apoplast juice from plant stem tissues
  • Method for separating apoplast juice from plant stem tissues
  • Method for separating apoplast juice from plant stem tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, isolating apoplast juice from Salicornia stem tissue

[0054] 1. Separation of apoplast juice

[0055] 1. Cut the 60-day-old Salthorn Grass from the 2-7cm area from the stem tip (such as figure 1 As shown in A), about 1g fresh weight. Subsequent operations were carried out at 4°C, in order to reduce the change of extrapopular body fluid components.

[0056] 2. Carefully cut out the stem tissue located between the outer cortex and the inner cortex (such as figure 1 As shown in middle B), cut it into strips with a length of 2.5 cm, and it is forbidden to cut the central column part (reason: the central column liquid will cause pollution of the apoplast juice).

[0057] 3. Arrange the strip-shaped tissue cut out, and wrap it lightly in the middle of the strip-shaped tissue with plastic wrap (the two ends of the strip-shaped tissue are not wrapped with plastic wrap), and place it on a plastic two-way tube from the top hole ( The diameter of the top hole is...

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Abstract

The invention discloses a method for separating apoplast juice from plant stem tissues. The method for separating apoplast juice from plant stem tissues comprises the following step: (1) putting segmented to-be-separated plant stem tissues to a two-way tube, so that the to-be-separated plant stem tissues are not leaked from the bottom hole of the two-way tube, wherein the to-be-separated plant stem tissues are stele-removed stem tissues; (2) putting the two-way tube which is contained with the to-be-separated plant stem tissues to a centrifugal tube, so that the top hole of the two-way tube corresponds to the tube port of the centrifugal tube; and (3) separating the apoplast juice from the to-be-separated plant stem tissues by centrifuging. The method provided by the invention can be widely applied to extracting apoplast juice of plants with deteriorated laves, and is of great meaning to finding the special substance exchange way of the plants.

Description

technical field [0001] The invention relates to a method for isolating apoplast juice from stem tissues of plants, in particular to a method for isolating apoplast juice from stem tissues of plants with degenerated leaves such as salicornia. Background technique [0002] Plant apoplast is composed of fibers and microcrystalline spaces outside the cell membrane that form the cell wall and intercellular spaces filled with water and air. The differentiated xylem also belongs to the apoplast[Sattelmacher B.The apoplast and its significance for plant mineral nutrition. New Phytologist, 2001, 149:167-192.]. It is a dynamic space in which the extracellular fluid is involved in many important physiological and biochemical processes, such as solute and water transport, homeostasis of the intracellular environment, growth and development, signal transduction, and resistance to adversity, etc. [Sakurai N. Dynamic function and regulation of apoplast in the plant body. Journal of Plant ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
Inventor 李银心陈显扬郭杰
Owner INST OF BOTANY CHINESE ACAD OF SCI
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