Cultivating method of laying hen with low egg yolk cholesterol content
A cultivation method and cholesterol technology, applied in the field of avian genetics and breeding, can solve the problems of labor, money, and slow breeding progress, and achieve the effects of reducing the cost of feeding, reducing the amount of feeding, and improving the accuracy of seed selection.
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[0024] Example 1
[0025] Screening of selected chicken samples
[0026] 1. Extraction of sample chicken DNA
[0027] In the basic breeding group, 500 sample chickens are selected and raised in a single cage, and the DNA of the sample chickens is extracted. The steps are as follows:
[0028] 1) DNA extraction: add 500μL TE buffer, SDS lysis solution (25%) 12μL, and proteinase K (10mg / L) 10μL to 80μL whole blood, shake and mix for 30min, digest in a water bath at 57℃ overnight; add Tris saturated phenol 602μL, shake for 10min, centrifuge at 10,000r / min for 10min; take the supernatant, add 500μL of Tris saturated phenol, shake for 10min, centrifuge at 10,000r / min for 10min; take the supernatant, add equal volume of phenol / chloroform / iso Amyl alcohol (25:24:1), shake slowly for 10min, centrifuge at 1,2000r / min for 10min; take the supernatant, add an equal volume of chloroform / isoamyl alcohol (24:1), shake slowly for 10min, 1,0000r Centrifuge the supernatant for 10 minutes; add 2 times ...
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[0060] Example 2
[0061] The sample chickens with the genotypes selected in Example 1 were selected for pure line breeding using the closed group breeding method, and 50 families were formed with a male-female ratio of 1:10. The individual cages were raised to 40 weeks of age, and the production of each hen was recorded. Egg number, average egg weight and egg yolk cholesterol content at 40 weeks of age, calculate the average value of 3 traits, egg weight, and egg yolk cholesterol content of all hens in 50 families and hens in each family, as shown in Table 5. Select traits based on egg yolk cholesterol content, taking into account egg weight and egg number, select the first 15 families whose egg yolk cholesterol content is lower than the group average, and the egg weight and egg production are higher than the group average, and breed the next generation, see Table 6 .
[0062] Table 550 The mean value of the 3 traits of all hens and hens of each family, egg weight, egg yolk chole...
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[0068] Example 3
[0069] The DNA of the 2-week-old offspring of the selected family chickens in Example 2 was extracted, and the pair of primers in Example 1 were used for PCR-RFLP analysis. According to the candidate individual's VLDLR gene molecular marker g.10428T> C, select the TT type for early molecular marker assisted selection.
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