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Freeze-dried vaccine against turkey herpesvirus type iii

A turkey herpes virus and vaccine technology, applied in the field of type III turkey herpes virus vaccine, can solve the problems of vaccine production and quality limitations, limited cell growth area, and difficult environmental conditions for cultivation, so as to achieve stable immune effect and infect cells The effect of high efficiency and small volume of production equipment

Active Publication Date: 2016-01-20
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main HVT vaccines on the market are basically obtained by using spinner flasks or shake flasks. The above-mentioned methods are labor-intensive, occupy a large space, and are easily polluted during the operation; the cell growth area provided per unit volume is limited, and the cell density is low; The environmental conditions are difficult to detect and control, and the batch-to-batch variation is large, which greatly limits the production and quality of vaccines.

Method used

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  • Freeze-dried vaccine against turkey herpesvirus type iii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation technology of embodiment 1 turkey herpes virus vaccine

[0035] The main steps of the preparation process for preparing turkey herpes virus freeze-dried live vaccine are as follows:

[0036] (1) Preparation of chicken embryo fibroblast (CEF) monolayer:

[0037] Select 10-day-old well-developed SPF chicken embryos, first use iodine tincture cotton balls to sterilize the air chamber, and then use 75% alcohol cotton balls to deiodine, aseptically pick out the embryo bodies and place them in sterile plates, and use 0.1M PBS Wash the embryo body and remove eyes, claws and viscera, then prepare CEF suspension by continuous digestion method to make a cell density of 800,000-1,200,000 / ml, mix well and inoculate the cell factory, 150-250ml per layer, 4% serum concentration M199 growth medium, cover the liquid inlet cap, put it in a 37°C incubator and culture it for 24-36 hours, and grow into a monolayer of cells for later use;

[0038] (2) Preparation of worki...

Embodiment 2

[0051] Embodiment 2 Turkey herpes virus immune effect comparison

[0052] Design experiment, comparatively utilize the qualified low-generation turkey herpes virus live vaccine of the preparation of embodiment 1 and the chicken Marek's disease turkey herpes virus live vaccine that provides with reference to the 2000 edition " Regulations of Veterinary Biological Products of the People's Republic of China " to manufacture And the immune effect of the turkey herpes virus live vaccine produced by the test procedure.

[0053] (1) Prepare seedlings according to the method of biological product regulations

[0054] Prepare the working virus seed according to the method of Example 1, then inoculate the spinner bottle, harvest the cells, freeze-dry after lyzing, and can be used after passing the test.

[0055] (2) immunity

[0056] 100 1-day-old SPF chickens provided by Beijing Merial Verton Experimental Technology Co., Ltd. were randomly divided into 4 groups, 20 in each group. Th...

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Abstract

The invention belongs to the technical field of biopharming, and discloses a method for preparing a chicken Marek's disease turkey herpes virus Fc126 strain live vaccine by utilizing a cell factory and the turkey herpes virus vaccine (Fc126 strain) prepared by utilizing the method. The turkey herpes virus vaccine (Fc126 strain) prepared in the cell factory is good in quality, high in immunization potency and stable in immunization effect. In addition, the turkey herpes virus vaccine (Fc126 strain) has the advantages of being low in generation, high in content of plaque forming unit (PFU), low in cost, simple in operation technology, easy to control and the like.

Description

technical field [0001] The invention relates to a type III turkey herpes virus vaccine, in particular to a type III turkey herpes virus freeze-dried vaccine. Background technique [0002] Type III turkey herpes virus (HVT) vaccine is an avirulent strain isolated from turkey flocks by Dr. WritterRL, an American scholar. In the vaccinated chicken body, the HVT in the cells can make the body produce antibodies, but cannot neutralize MDV. This vaccine has an interfering effect. The wide application of the vaccine can effectively resist the virulence. It can exist in the form of cell binding, and a small amount can be free outside the cell. Its advantage is that it can be made into a freeze-dried vaccine that is easy to store and transport. Since the 1970s, HVT vaccine has played a huge role. [0003] Considering that the HVT vaccine is easy to store, transport and use, and the cost is low, the HVT vaccine is still used to prevent the outbreak of MDV in areas with good sanita...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/245A61K9/19A61P31/22
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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