Application of bactericidal/permeability-increasing protein gene in serving as common disease resistance genetic marker of pigs

Abstract

The invention relates to a genetic marker for screening the combined gene model of the fourth and tenth exon variation sites of a common disease resistance BPI (bactericidal / permeability increasing protein) gene of pigs, and belongs to the technical fields of genetic breeding and molecular marker assisted selection of pigs. The method comprises the following steps of: extracting DNA (deoxyribonucleic acid) of a pig genome of a sample to be tested, and performing PCR (polymerase chain reaction) amplification on the pig genome; analyzing the PCR product of the exon 4 through SSCP (single strand conformation polymorphism), and performing enzyme digestion on the PCR product of the exon 10 through restriction enzyme HpaII, wherein when the gene model of the pig body to be tested is a CDAB model, the pig body is strong in common disease resistance. According to the application, a molecular genetic marker with higher efficiency and accuracy is provided to molecular marker assisted selection of the pig disease resistance breeding work, and an effective molecular marker breeding means is provided for improving the common disease resistance and immune response capability of piglets. The detection method is simple in operation, low in cost and high in accuracy, can realize automatic direct detection, and takes a magnificent effect in pig breeding.

Description

technical field [0001] The invention belongs to the technical field of pig genetic breeding and molecular marker-assisted selection, and specifically relates to the screening and application of pig general disease resistance genetic markers. Background technique [0002] With the popularization of intensive feeding and management methods, in the production of pig industry, the hazards of diseases can easily bring heavy losses to animal husbandry production. In the United States, swine diseases cost more than $1.5 billion annually. This huge economic loss is mainly due to pig deaths, reduced production efficiency, increased medical expenses, and product loss. Under stressful production conditions, selective pressure on economically important traits can also lead to increased disease incidence. Diseases can reduce the efficiency of selection and affect the genetic improvement of production traits. At present, the control measures for livestock and poultry diseases mainly us...

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Problems solved by technology

[0008] There are few reports on the polymorphism of the pig BPI gene and its resistance / susceptibility to diseases at home and abroad. There is a sequence of the pig BPI gene in the US Patent Network, which is 1452bp long and has no clear annotation. Now Name it the patent BPI sequence (United States, Kind Code: A1, Patent Application: 20040234980)

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