Marking method of applying SSAP molecular marker technology to peony
A technology of molecular markers and marker methods, applied in the field of molecular biology, can solve the problems of large heterogeneity and numerous steps of retrotransposon LTR sequences
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[0104] A kind of labeling method of SSAP molecular labeling technology on peony, comprises the following steps:
[0105] Step 1. Design primers
[0106] Design Mse I and EcoR I linker primers, pre-amplification PCR primers, linker selective amplification PCR primers, and retroposon PCR primers. The primer sequence direction is from 5' to 3':
[0107] 1) Linker primers
[0108] Mse I Adapter Primer
[0109] Mse I 5′ end adapter primer: Mzx5 GACGATGAGTCCTGAG
[0110] Mse I 3′ end adapter primer: Mzx3 TACTCAGGACTCAT
[0111] EcoR I Adapter Primer
[0112] EcoRI 5′ end adapter primer: Ezx5 CTCGTAGACTGCGTACC
[0113] EcoRI 3′ end adapter primer: Ezx3 AATTGGTACGCAGTCTAC
[0114] 2) Pre-amplification primers
[0115] Mse I preamplification primer: Mzx00 GATGAGTCCTGAGTAAC
[0116] EcoR I preamplification primer: Ezx00 GACTGCGTACCAATTCA
[0117] 3) Selective amplification primers
[0118] Adapter selection amplification primers:
[0119] Mzx02: GATGAGTCCTGAGTAACAT
[0120] Mz...
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