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L-lactic acid production method and special Bacillus sp. therefor

The technology of Bacillus sp. and Bacillus fermentum is applied in the field of special Bacillus sp. strains, which can solve problems such as failure to meet industrialization requirements, and achieve the effects of simplifying post-processing processes, reducing fermentation costs, and reducing environmental pollution.

Active Publication Date: 2014-07-16
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is only one report abroad on the production of lactic acid by fermentation of alkaliphilic bacteria (Calabia et al., Biotechnol. Lett., 2011, 33: 1429-1433), but in this report, the yield, conversion rate and optical purity of L-lactic acid are only 65.8 g / L, 83% and 98.8%, not up to industrial requirements

Method used

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  • L-lactic acid production method and special Bacillus sp. therefor

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Experimental program
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Effect test

Embodiment 1

[0030]Example 1, Isolation and Identification of Bacillus sp. WL-S20

[0031] 1. Separation of WL-S20

[0032] Screening medium (g / L): glucose 10.0, yeast powder 5.0, polypeptone 5.0, K 2 HPO 4 ·3H 2 O 1.0, MgSO 4 ·7H 2 O 0.2, NaCl 50, distilled water 900ml, high temperature sterilization at 115°C for 20 minutes, then add high temperature sterilized 10% (w / v) Na 2 CO 3 100ml. For solid medium, 0.1-0.2 g / L agar needs to be added.

[0033] Mud samples were obtained from the edge of the Ordos Saline-Alkali Lake in Inner Mongolia. Weigh 5g of mud sample into a 250ml Erlenmeyer flask, add 100ml of screening medium, mix thoroughly, dilute 10,000 times with screening medium and spread on solid screening medium, culture at 37°C for 3 days, and inoculate the grown single colony into A test tube containing 4ml of selection medium was cultured statically at 37°C for 24 hours. At the end of the culture, take the fermentation broth, centrifuge at 6,000 rpm with a centrifugal rad...

Embodiment 2

[0044] Embodiment 2, the application of Bacillus sp. WL-S20 CGMCC NO.5634

[0045] method one:

[0046] 1. Fermentation

[0047] The composition of the medium used in this embodiment is as follows:

[0048] Seed medium (g / L): glucose 10.0, yeast powder 5.0, polypeptone 5.0, K 2 HPO 4 ·3H 2 O 1.0, MgSO 4 ·7H 2 O 0.2, NaCl 50, distilled water 900ml, high temperature sterilization at 115°C for 20 minutes, then add high temperature sterilized 10% (w / v) Na 2 CO 3 100ml.

[0049] Fermentation medium (g / L): Glucose 80, peanut meal (Beijing Kangmingwei Medium Technology Co., Ltd.) 20, MgSO 4 ·7H 2 O 0.35, K 2 HPO 4 ·3H 2 O2,CH 3 COONa 1, MnSO 4 ·H 2 O 0.03. Sterilize at 115°C for 20 minutes.

[0050] The method for producing L-lactic acid by fermentation method of the present invention comprises the following steps:

[0051] 1), activation culture: the preserved Bacillus sp. (Bacillus sp.) WL-S20 CGMCC NO.5634 glycerol bacterium liquid is inoculated into a test tube ...

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Abstract

The invention discloses an L-lactic acid production method and special Bacillus sp. therefor. The Bacillus sp. WL-S20 provided in the invention has a preservation number of CGMCC NO.5634 and can be used for preparing L-lactic acid. Experiments in the invention prove that the Bacillus sp. WL-S20 having a preservation number of CGMCC NO.5634 can be used for producing L-lactic acid, L-lactic acid having an optical purity close to 100% is produced through the efficient fermentation at 45DEG C in a conversion rate of 98.6-99.3% by treating glucose as a substrate, and the highest concentration of L-lactic acid can reach 225g / L.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing L-lactic acid and special bacillus strains thereof. Background technique [0002] Lactic acid, also known as dihydroxypropionic acid, is one of the three major organic acids in the world. As a traditional multi-purpose fine chemical, lactic acid can be used as sour agent, fragrance, preservative, plant growth regulator, biodegradable material, drug and pesticide, etc., used in food, pharmaceutical, brewing, tanning, Textile, environmental protection and agriculture. Lactic acid is a chiral molecule and can be divided into L-lactic acid, D-lactic acid and DL-lactic acid. L-lactic acid is the raw material for producing poly-L-lactic acid. The poly-L-lactic acid produced by the polymerization of L-lactic acid is recognized as one of the ideal biodegradable plastics to replace traditional plastics because of its good biodegradability and excellent use characteri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/07
Inventor 马延和孟莹薛燕芬于波
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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