An immunoliposome biochip, its preparation method and its application in biological detection

A technology of immunoliposome and plastid biology, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of inappropriate high-throughput analysis and time-consuming, and achieve the effect of reducing dosage and improving binding ability.
CN103197066BActive Publication Date: 2015-12-23美国纳米材料创新有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
美国纳米材料创新有限公司
Publication Date
2015-12-23

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Abstract

The invention relates to an immunoliposome biochip, a preparation method thereof and an application thereof in biological detection. The immunoliposome provided by the invention is distributed on the chip in a micro-array way, and includes molecule probes, such as molecular beacon with fluorescence; and outside of the immunoliposome is loaded with various ligands. The immunoliposome biochip can specifically capture target cells in a cell mixture, and ensure messenger RNA and / or micromolecule RNA in the target cells without destroying biomarkers in the cells. In addition, the immunoliposome biochip provided by the invention can be used for capturing and selecting nanoparticles secreted by virus and cells, such as micro bubbles and exosomes. The immunoliposome biochip has advantages of simple operation, low cost, rapidness, sensitive and high selectivity, and can be used for molecular detection of various diseases, food safety and other fields.
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Description

technical field

[0001] The invention relates to an immunoliposome biochip, and at the same time, the invention also relates to a preparation method of the immunoliposome biochip and its application in biological detection. Background technique

[0002] Antibodies and other ligands on the cell surface have been widely used in the field of bioanalytical detection, such as by magnetic activated cell sorting (MACS), in which ligands are immobilized on magnetic nanoparticles; in addition, such as by fluorescence activated cell sorting ( FACS), in which fluorochrome-labeled antibodies are used for cell isolation, however, fluorochrome-dyed antibodies and magnetic nanoparticles for immobilizing antibodies are expensive and time-consuming. For the separation of mixed cells, cell chip sorting methods based on solid substrates are the development trend in recent years, because they are convenient to immobilize various surface ligands for screening cells, such as antigens, and have lig...

Claims

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