Kit for detecting deaf related mitochondrial T7505C mutation, and application thereof

A technology of T7505C and mitochondria, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as troubles, and achieve the effects of relieving pain, simple detection process, and intuitive result interpretation
CN103266169BActive Publication Date: 2015-04-08ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2015-04-08

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Abstract

The invention provides a kit for detecting deaf related mitochondrial DNA T7505C mutation. The kit is composed of a DNA extraction mixed liquid, a PCR mixed liquid for T7505C fragment amplification, a pair of outer primers designed against T7505C, a pair of inner primers designed against the T7505C, a restrictive endonuclease, a positive contrast, a negative contrast and a kit body. A protease K digestion cracking method is utilized in the invention to rapidly extract genome DNA from a small amount of specimens, so a problem of the traditional extraction of DNA from the blood of a deaf patient is solved, the pains of a detected person is mitigated, and a trans-regional sample transmission problem is also solved. The kit has the advantages of low application cost, simple and rapid detection flow, visual result interpretation, ensuring of the specificity and the stability of the detection result, and realization of the application in the detection of the deaf related mitochondrial tRNAGlnT7505C mutation.
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Description

technical field

[0001] The invention belongs to the field of biological technology, and relates to a kit for detecting the mitochondrial DNA T7505C mutation related to deafness, and also relates to a detection method for the mitochondrial gene mutation related to deafness, in particular to detecting mitochondrial tRNA Ser(UCN) The method for the T7505C mutation, and the application of the above-mentioned method or the above-mentioned kit in detecting the mitochondrial DNA T7505C mutation related to deafness. Background technique

[0002] Deafness is a common disabling disease that causes speech communication barriers and is one of the greatest sufferings of human beings. According to WHO estimates in 2005 that there were 278 million hearing disabled people in the world, accounting for 4.6% of the world's total population; 80% of hearing disabled people live in low- and middle-income countries. Deafness has become a serious health and safety problem in my country, bringing ...

Claims

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