Articles and method for detecting a target microorganism
A technology of microorganisms and indicator microorganisms, applied to the microbial content of samples (environmental samples), to detect the presence or absence of target microorganisms, and to solve problems such as high cost
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Embodiment 1
[0111] Embodiment 1 is a method for detecting the presence or absence of a target microorganism, comprising:
[0112] supply:
[0113]a culture device comprising a culture medium containing ingredients selected to promote growth of predetermined indicator microorganisms;
[0114] a detection article comprising a first indicator system selected to detect a target microorganism; and
[0115] sample;
[0116] inoculating the culture device with the sample;
[0117] incubating said inoculated culture device for a period of time sufficient to allow growth of said indicator microorganism;
[0118] observing the culture device for at least one indication indicating the presence of microorganisms;
[0119] contacting the medium of the incubated culture device with the test article; and
[0120] The culture device contacted with the article is observed to detect a transition of the first indicator system from the first state to the second state.
Embodiment 2
[0121] Embodiment 2 is the method of embodiment 1, wherein if there is a transition of the first indicator system from the first state to the second state, the presence of at least one target microorganism is indicated.
Embodiment 3
[0122] Embodiment 3 is the method of embodiment 1, wherein the absence of a transition of the first indicator system from the first state to the second state indicates the presence of at least one target microorganism.
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