Method for preparing compound enzyme and probiotic preparation for feed by biotransforming sorghum straw and rapeseed cake

Abstract

The invention discloses a method for preparing a compound enzyme and probiotic preparation for feed through the biotransformation of sorghum straws and rapeseed meals. The method comprises the steps of: thoroughly mixing cooked Pu'er tea, mushroom sticks, tea tree mushroom sticks, first sorghum straws, first rapeseed meals, first wheat bran, first apple pomace and first water which are subjected to pile fermentation, and carrying out acclimation culture so as to form a leavening agent; and thoroughly mixing the leavening agent, second sorghum straws, second rapeseed meals, second wheat bran, second apple pomace and second water, and carrying out fermentation, thereby obtaining the compound enzyme and probiotic preparation for feed. According to the method, the compound enzyme and probiotic preparation with better various performance indexes is prepared through fermenting the sorghum straws and the rapeseed meals by using food-grade safe flora with stable microbial states, so that the low-cost and high-efficiency biotransformation of the sorghum straws and the rapeseed meals is realized. Meanwhile, the method disclosed by the invention has the advantages that microbes grow easily, the enzyme activity is high, the fermentation process is extensive, and the industrial large-scale production is facilitated.

Description

technical field

[0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting sorghum stalks and rapeseed cakes with micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique

[0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed.

[0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellu...

Examples

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu-erh cooked tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of sorghum straw, 5kg of rapeseed cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0028] 2) Fully mix 20kg of starter in step 1) with 600kg of sorghum stalks, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 77.23IU / g; total cellulase activity reaches 6.88IU / g; beta-glucosidase activity reaches 77.23IU / g; reached 15.56IU / g; endoglucanase activity reached 55.90...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of sorghum straw, 5kg of rapeseed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0032] 2) Fully mix 20kg of starter in step 1) with 600kg of sorghum stalks, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 76.78IU / g; total cellulase activity reaches 6.96IU / g; beta-glucosidase activity reaches 76.78IU / g; reached 15.42IU / g; endoglucanase activity reached 55.46IU / g; x...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu’er tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of sorghum straw, 5kg of rapeseed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 36 hours of acclimatization and cultivation, a leavening agent is formed;

[0036] 2) Fully mix 10kg of starter in step 1) with 600kg of sorghum straw, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and fermented at 35°C After 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 74.92IU / g; total cellulase activity reaches 6.14IU / g; beta-glucosidase activity reaches 74.92IU / g; reached 15.51IU / g; endoglucanase activity reached 52.3...

technical field

[0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting sorghum stalks and rapeseed cakes with micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique

[0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed.

[0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellu...

Examples

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu-erh cooked tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of sorghum straw, 5kg of rapeseed cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0028] 2) Fully mix 20kg of starter in step 1) with 600kg of sorghum stalks, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 77.23IU / g; total cellulase activity reaches 6.88IU / g; beta-glucosidase activity reaches 77.23IU / g; reached 15.56IU / g; endoglucanase activity reached 55.90...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of sorghum straw, 5kg of rapeseed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0032] 2) Fully mix 20kg of starter in step 1) with 600kg of sorghum stalks, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 76.78IU / g; total cellulase activity reaches 6.96IU / g; beta-glucosidase activity reaches 76.78IU / g; reached 15.42IU / g; endoglucanase activity reached 55.46IU / g; x...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu’er tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of sorghum straw, 5kg of rapeseed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 36 hours of acclimatization and cultivation, a leavening agent is formed;

[0036] 2) Fully mix 10kg of starter in step 1) with 600kg of sorghum straw, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and fermented at 35°C After 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 74.92IU / g; total cellulase activity reaches 6.14IU / g; beta-glucosidase activity reaches 74.92IU / g; reached 15.51IU / g; endoglucanase activity reached 52.3...