Domestication cultivation method of bletilla tissue culture seedlings

A cultivation method, technology of Bletilla striata group, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of poor self-support ability, absorption function, stalk guidance and transportation function, leaf photosynthesis function and inability to adapt to the outside world environment, low reproduction coefficient and other issues, to achieve the effect of improving the survival rate of transplanting, quickly adapting to the natural environment, and improving the absorption function

Inactive Publication Date: 2013-09-25
重庆市秀山红星中药材开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the current conventional methods are based on division, that is, the tubers are cut into small pieces for planting, and the reproduction coefficient is low, which can no longer meet the needs of the market.
People have started artificial propagation, and many places have adopted the method of tissue culture propagation to obtain a large number of seedlings quickly; because tissue culture propagation is carried out in an artificial environment, its self-supporting ability is very poor, especially the absorption function of its roots, stems, etc. The dredging and transportation function of the leaves and the photosynthetic function of the leaves cannot adapt to the external environment, and they cannot fully perform their functions, which leads to a very low survival rate of transplanting. Therefore, domestication is required to successfully transplant to improve its survival. Rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] First prepare the domestication matrix, crush 1 ton of perlite, 3 tons of peat soil, and 1 ton of silica into particles with a particle size below 2mm, and then mix them evenly with 0.5 tons of ternary compound fertilizer with a nitrogen, phosphorus, and potassium content of 10:10:10 , that is, to make the domestication matrix of tissue culture seedlings.

[0017] Move the tissue-cultured seedlings with a plant height of 3-4cm and more than 5 root fibers together with the seedling bottle to the seedling hardening room for seedling hardening. After 4 days in the room under natural light, open the bottle cap and leave it for 2 days to allow air to come in and out freely. ; Then inject a small amount of tap water into the seedling bottle, gently take it out together with the medium with tweezers, put it in a clean water basin to wash the medium, and then rinse it gently with tap water; then transplant the rinsed tissue culture seedlings into the greenhouse Bletilla striata...

Embodiment 2

[0019] First prepare the domestication matrix, crush 1 ton of perlite, 3 tons of peat soil, and 1 ton of silica into particles with a particle size below 2mm, and then mix them evenly with 0.5 tons of ternary compound fertilizer with a nitrogen, phosphorus, and potassium content of 10:10:10 , that is, to make the domestication matrix of tissue culture seedlings.

[0020] Move the tissue-cultured seedlings with a plant height of 3-4cm and more than 5 root fibers together with the seedling bottle to the seedling hardening room for seedling hardening. After 4.5 days in the room under natural light, open the bottle cap and place it for 2 and a half days to let the air freely. In and out; then pour a small amount of tap water into the seedling bottle, gently take it out together with the medium with tweezers, put it in a clean water basin to wash the medium, and then rinse it gently with tap water; then transplant the rinsed tissue culture seedlings to the greenhouse Bletilla stria...

Embodiment 3

[0022] First prepare the domestication matrix, crush 1 ton of perlite, 3 tons of peat soil, and 1 ton of silica into particles with a particle size below 2mm, and then mix them evenly with 0.5 tons of ternary compound fertilizer with a nitrogen, phosphorus, and potassium content of 10:10:10 , that is, to make the domestication matrix of tissue culture seedlings.

[0023] Move the tissue-cultured seedlings with a plant height of 3-4cm and more than 5 root fibers together with the seedling bottle to the hardening room for hardening. After 5 days in the room under natural light, open the bottle cap and leave it for 3 days to allow air to come in and out freely. ; Then inject a small amount of tap water into the seedling bottle, gently take it out together with the medium with tweezers, put it in a clean water basin to wash the medium, and then rinse it gently with tap water; then transplant the rinsed tissue culture seedlings into the greenhouse Bletilla striata tissue-cultivated...

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Abstract

The invention discloses a domestication cultivation method of bletilla tissue culture seedlings, which belongs to the technical field of Chinese herbal medicine planting. The method comprises the following five steps: matrix preparation, hardening-seedling, bottling-out, domestication cultivation, and domestication management. The method has the beneficial effects that a method for transiting bletilla tissue culture seedlings from an experiment bottle to the nature is provided, so that the absorption function of root hairs, the guided transport function of stems and the photosynthesis function of leaves of tissue culture seedlings can be quickly improved, and therefore, the tissue culture seedlings can adapt to the natural environment quickly, the transplanting survival rate of tissue culture seedlings is improved, and by using the method, the transplanting survival rate of domestication seedlings reaches over 92%.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, in particular to a method for domesticating and cultivating bletilla striata tissue culture seedlings. Background technique [0002] Bletilla striata is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae, also known as white root, ground screw, white chicken baby, horn seven, purple orchid, knife-edge medicine, Lianjicao, etc. The tubers are used for medicine, which can restrain bleeding, reduce swelling and promote granulation; treat diseases such as tuberculosis and hemoptysis, bronchiectasis hemoptysis, gastric ulcer, hematemesis, hematuria and hematochezia. External use to treat traumatic bleeding, burns, chapped hands and feet embolism. It has huge commercial application value in biomedicine, health food, textile printing and dyeing, special coatings and daily chemical industry. With the development and utilization of bletilla ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨再江陈荣袁志强万联新邹代学
Owner 重庆市秀山红星中药材开发有限公司
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