Device and method for detecting existence of target biomolecules in a specimen

Abstract

A detecting device is used for detecting existence of target biomolecules in a specimen with use of antibody complexes labeled with fluorescent molecules. The detecting device includes a capture member coated with capture antibodies for immobilizing the antibody complexes on the capture member when the target biomolecules exist in the specimen, a light emitting unit emitting a beam for exciting the fluorescence molecules to generate a fluorescence signal, and a signal processing unit for receiving the fluorescence signal and determining existence of the target biomolecules in the specimen based upon receipt of the fluorescence signal.

Description

technical field [0001] The invention relates to a measurement technology, in particular to a measurement device and method for judging whether a target biomolecule exists in a sample to be tested. Background technique [0002] refer to figure 1 and figure 2 , in Chinese Taiwan Patent I342389, a measuring device for judging whether a target biomolecule (such as an antigen) exists in a test sample 1 is proposed in the past. The measurement device includes a laser light source 21 , an optical chopper 22 (chopper), a lens 24 , a multimode optical fiber 14 and a signal receiving and processing unit 23 . [0003] The sample 1 to be tested has two states. One of the states is that the target biomolecule exists in the sample 1 to be tested. At this time, the sample 1 to be tested includes a plurality of antibodies 11 immobilized on the surface of the multimode optical fiber 14, and a plurality of antibodies 11 for the target biomolecule. Biomolecules 12, and a plurality of anti...

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Problems solved by technology

[0005] In the above method, after most of the second incident light 202 enters the multimode optical fiber 14, it is totally reflected inside and generates evanescent waves, and the fluorescent molecules are excited by the localized surface plasmon electric field of the metal nanoparticles 133 132 produces intensity-modulated fluorescent signals, which have high intensity noise in the detection of fluorescent signals, so there is a certain limit in detection sensitivity

The evanescent wave is generated by the total reflection inside the multimode fiber 14, which is localized and the electromagnetic field strength is attenuated, so it is not an efficient way to excite fluorescent signals.

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