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Amphora coffeaeformis hypnospore compounding agent for stichopus japonicus seaculture and preparation method thereof

A technology of Dimenorrhea coffea, dormant spores, applied in the directions of methods using spores, microorganism-based methods, biochemical equipment and methods, etc.

Inactive Publication Date: 2013-11-20
QINZHOU XIAXIEBAO BAIT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The object of the present invention is to provide a dormant spore complexing agent for A. japonicus in seawater cultivation of A. japonicus and a preparation method thereof, so as to solve the problem of directional propagation of diatoms for the cultivation of A. japonicus in ponds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A kind of A. japonicus seawater cultivation can replace the dormant spore compounding agent of A. coffee-shaped double browed algae, calculated in parts by weight as follows:

[0040] Take 1 part of dormant spores of Amygdalina coffee-shaped, 0.30 parts of ferric citrate, 9.6 parts of ferric chloride, 8 parts of sodium metasilicate, 0.60 parts of disodium EDTA, 10.40 parts of vitamin B, vitamin B 12 0.10 parts; 80 parts of diammonium phosphate.

[0041] The preparation method is to make the matrix and nutrient base by batching, pulverizing and mixing the raw materials, and the specific operation steps are as follows:

[0042] (1) Take ferric citrate, ferric chloride, sodium metasilicate, disodium EDTA, vitamin B1, vitamin B12 according to the proportioning scale, and crush them; add the dormant spores of Amphitheater spp., mix and stir for 12 minutes to prepare matrix,

[0043] (2) Diammonium phosphate is weighed according to the proportion, crushed, and made into a n...

Embodiment 2

[0047] Another example of the present invention, a sea cucumber mariculture can replace the dormant spore complexing agent of coffee-shaped biciliated algae, and its formula ratio is calculated according to the following parts by weight:

[0048] Get 0.80 parts of dormant spores of Amphipena caffeifera, 0.20-parts of ferric citrate, 8 parts of ferric chloride, 6 parts of sodium metasilicate, 0.50 parts of disodium EDTA, 0.06 parts of vitamin B, Vitamin B120.08 parts; diammonium phosphate 84.42 parts. The preparation method and the technical conditions of the dormant spore complexing agent of the sea-cultured ginseng sea cucumber are the same as those in Example 1.

Embodiment 3

[0050] Yet another example of the present invention, a kind of C. chinensis mariculture can replace the dormant spore complexing agent of coffee-shaped Amphetella amphitidis, and its formula ratio is counted as follows in parts by weight:

[0051] Get 1.2 parts of coffee-shaped Amphora dry powder, 0.40 parts of ferric citrate, 12 parts of ferric chloride, 10 parts of sodium metasilicate, 0.70 parts of disodium EDTA, 10.10 parts of vitamin B10, and 0.12 parts of vitamin B12; 75.58 parts of diammonium phosphate. The preparation method and the technical conditions of the dormant spore complexing agent of the sea-cultured ginseng sea cucumber are the same as those in Example 1.

[0052] The present invention uses effect as follows:

[0053] It was tested in a ginseng pool on Weizhou Island, Beihai, Guangxi, with an area of ​​5.5 mu, including 40 square meters of artificial ginseng reef, and an average water depth of 1.7 meters. Before the seedlings are placed, the water quality ...

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Abstract

The invention relates to a Amphora coffeaeformis hypnospore compounding agent for stichopus japonicus seaculture and a preparation method thereof. The compounding agent comprises a substrate and a nutrient medium. The preparation method comprises the following steps: (1) mixing 0.80-1.20 parts by weight of Amphora coffeaeformis hypnospore, 0.20-0.40 parts of ferric citrate, 7.94-11.9 parts of iron chloride, 6-10 parts of sodium metasilicate, 0.50-0.70 part of disodium EDTA, 0.06-0.10 part of vitamin B1 and 0.08-0.12 part of vitamin B12, and stirring to obtain the substrate; and (2) weighing 84.42-75.58 parts of diammonium phosphate, pulverizing to obtain the nutrient medium, and mixing 15.58-24.42 parts of substrate and 84.42-75.58 parts of nutrient medium diammonium phosphate to obtain the compounding agent. The invention has the action of orientated reproduction of Amphora coffeaeformis, and can subsitute a stichopus japonicus seaculture feed.

Description

technical field [0001] The invention relates to a dormant spore complexing agent for A. japonicus and a preparation method thereof, belonging to the technical field of aquaculture. Background technique [0002] Radix ginseng is the first treasure of the four treasures of my country's food "ginseng, abalone and swallow's wings". Stichopus japonicus, taxonomic status is: Animal Kingdom, Echinodermata, Echinacea, Sea Cucumber, Subclass Subclass, Subclass, Order Aposticidae. [0003] A. coffee eformis (Ag.) kuetzing. The taxonomic status of A. coffee eformis (Ag.) kuetzing. The taxonomic status is: Plantae, Diatomphyta, Pleurophyta, Navicales, Bridge Curvaginaceae, A. coffeaeformis, Coffeae. [0004] Traditional aquaculture is mainly based on feeding feed, and now it has formed a huge production scale, supporting my country's status as the world's largest aquaculture country. However, with the continuous development and expansion of the production of feeding feed, the problem ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N3/00A01K61/00C12R1/89
CPCY02A40/81
Inventor 赖朝钦
Owner QINZHOU XIAXIEBAO BAIT
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