Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer

A technology for rice sheath blight and bio-organic fertilizer, which is applied in the field of microorganisms and achieves remarkable effects

Active Publication Date: 2013-11-20
南京三美农业发展有限公司
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  • Application Information

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Problems solved by technology

[0007] So far, no literature reports related to the subject of the present invention have been found

Method used

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  • Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer
  • Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer
  • Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer

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Embodiment 1

[0036] (1) Isolation and identification of strains

[0037] Samples of poorly ventilated soil, beach silt soil, and diving silt were collected and stored at low temperature. Martin's medium was used to isolate fungi, and confrontation culture was used to isolate strains that had an inhibitory effect on the rice sheath blight pathogen Rhizoctonia solani. Biological control strains can completely inhibit the growth of Rhizoctonia solani in 72 hours ( figure 2 ).

[0038] The above-mentioned Martin’s medium preparation method is (take the preparation of 1L medium as an example): 5g of peptone, 10g of glucose, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.5g, 20g agar, 1000ml water, natural pH, 3.3ml 1% Bengal red aqueous solution, sterilize at 115°C for 30 minutes, add 0.3ml 1% streptomycin solution to every 100ml medium before use.

[0039] The biological control strain belongs to Trichoderma aculeatus ( T. asperellum ), the main biological characteristics are: cultured on PDA medium at...

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Abstract

The invention discloses a biological prevention and control bacterial strain for preventing and controlling rice sheath blight, a biological organic fertilizer, and a preparation method of the biological organic fertilizer, which relate to biological prevention and control bacterial strains of rice and biological organic fertilizers thereof, and belong to the technology of intensive agricultural production. According to the invention, Trichodermaasperellum12, which plays an obvious role in biological prevention and control on the rice sheath blight, is obtained by separation, the biological organic fertilizer is prepared by composting the biological prevention and control bacterial strain, rice hulls, straws and poultry excrement and contains bacterial strain hypnospore of more than 0.5*10<8>cfug<-1>, the water content of the fertilizer is smaller than 15% and the organic content of the fertilizer is more than 35%. Experiments show that after the biological organic fertilizer is applied into rice fields, most of matter in the organic fertilizer can keep afloat, the hypnospore of the biological prevention and control bacterial strain, which is adsorbed on the organic fertilizer, can rapidly breed on water, aerial mycelium is formed at a water-gas junction zone, and a hypha belt is formed at the contact part of rice stalks and the water surface, so that rhizoctonia solani of the rice sheath blight on the water surface is effectively prevented from contacting the rice stalks, and the prevention and control rate of the rice sheath blight is more than 70%.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to the isolation and identification of a rice biological control bacterial strain, the bio-organic fertilizer prepared by the biological control bacterial strain and its application in the prevention and treatment of rice sheath blight. 2. Background technology [0002] Rice is the crop with the largest planting area in the world. Its planting area reaches 150 million hectares, accounting for 11% of the world's cultivated land area. The planting area of ​​rice in my country accounts for more than 20% of all crops. It is the main food crop in my country and the first Rice is the staple food of more than 40% of the population. [0003] Rice sheath blight is one of the main diseases of rice. At present, the diseased area and damage loss rank first among rice diseases. Generally, rice production can be reduced by 10%-30%, and in severe cases, it can be reduced by 50%. In addition to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05G3/00A01P3/00C12R1/885
Inventor 陈立华邵孝侯毛欣宇朱福新谈俊益崔智伟王莎莎
Owner 南京三美农业发展有限公司
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