Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer
A technology for rice sheath blight and bio-organic fertilizer, which is applied in the field of microorganisms and achieves remarkable effects
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[0036] (1) Isolation and identification of strains
[0037] Samples of poorly ventilated soil, beach silt soil, and diving silt were collected and stored at low temperature. Martin's medium was used to isolate fungi, and confrontation culture was used to isolate strains that had an inhibitory effect on the rice sheath blight pathogen Rhizoctonia solani. Biological control strains can completely inhibit the growth of Rhizoctonia solani in 72 hours ( figure 2 ).
[0038] The above-mentioned Martin’s medium preparation method is (take the preparation of 1L medium as an example): 5g of peptone, 10g of glucose, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.5g, 20g agar, 1000ml water, natural pH, 3.3ml 1% Bengal red aqueous solution, sterilize at 115°C for 30 minutes, add 0.3ml 1% streptomycin solution to every 100ml medium before use.
[0039] The biological control strain belongs to Trichoderma aculeatus ( T. asperellum ), the main biological characteristics are: cultured on PDA medium at...
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