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Method for culturing photosynthetic bacteria

A technology of photosynthetic bacteria and culture, applied in the field of large-scale cultivation of photosynthetic bacteria with high cell concentration, can solve the problems of photosynthetic bacteria growth rate reduction, affecting enzyme stability, enzyme destruction, etc., to achieve continuous rapid growth, high-efficiency cultivation and production, and high Effect of High Cell Concentration

Inactive Publication Date: 2010-10-27
广东绿百多生物科技有限公司 +1
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AI Technical Summary

Problems solved by technology

The optimum pH for the cultivation of photosynthetic bacteria is around 7.0, too high or too low a pH value will affect the stability of the enzyme, and then destroy the enzyme, resulting in a decrease in the growth rate of photosynthetic bacteria

Method used

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  • Method for culturing photosynthetic bacteria
  • Method for culturing photosynthetic bacteria
  • Method for culturing photosynthetic bacteria

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Embodiment Construction

[0013] Firstly, in the cement pool constructed with a length of 5 meters, a width of 4 meters and a height of 0.3 meters, tap water was used to prepare a liquid culture medium, and its composition was (g / L): NH 4 Cl1.5, KH 2 PO 4 1.5, MgSO 4 7H 2 O0.8, NaCl4.0, NaHCO 3 1.8, CaCl 2 0.01, VB0.1, yeast powder 1.0, sodium acetate 4.0, trace element solution 1.0ml / L. Trace element solution includes (mg / L): FeCl 3 6H 2 O5.0, CuSO 4 5H 2 O0.05, H 3 BO 3 1.0, MnCl 2 4H 2 O0.5, ZnSO 4 7H 2 O1.0, CoCl 2 6H 2 O0.5. After preparing 3500 liters of photosynthetic bacteria medium in the cement pool according to the formula, inoculate 525 liters of cultured Rhodopseudomonas palustris liquid according to the ratio of 15%, and cover the surface of Rhodopseudomonas palustris culture with a transparent plastic film. Cultivate under sunlight. During the cultivation process, samples were taken at 10 o'clock in the morning every day to detect the pH of the Rhodopseudomonas palustr...

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Abstract

The present invention provides a method for culturing photosynthetic bacteria, which belongs to the biotechnology field. The method adopts a large cement pool as a culturing container and uses a transparent plastic film to cover and seal the surface of liquid photosynthetic bacteria culture in the cement pool, and the photosynthetic bacteria are cultured under the irradiation of sunlight with average temperature larger than 20 DEG C. After culturing is started, the photosynthetic bacteria culture is sampled everyday in order to measure the pH of the photosynthetic bacteria culture, and by adding acetic acid solution with concentration between 5 percent and 30 percent into the photosynthetic bacteria culture and uniformly mixing, the pH of the photosynthetic bacteria culture is decreased to 7.0. The method can culture the photosynthetic bacteria on a large scale and promote the photosynthetic bacteria to grow rapidly and constantly, the dry-weight concentration of the finally obtained Rhodopseudomonas palustris cells is 1.8g / L, and the corresponding cell concentration reaches 81.8 multiplied by 10<8> / ml. The method realizes the purpose of rapidly and efficiently culturing high-cell-concentration photosynthetic bacteria on a large scale.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular provides a method for large-scale cultivation of photosynthetic bacteria with high cell concentration. Background technique [0002] Photosynthetic bacteria (Photosynthetic Bacteria) is a general term for a large class of prokaryotic organisms that can perform non-oxygenous photosynthesis under anaerobic light conditions. ), Chlorobiaceae (Chlorobiaceae) and green filamentous fungi (Chloroflexaceae) (Chloroflexaceae) 4 families, bacterial cells are mainly divided into rod-shaped and spherical two different forms, is one of the pioneer species of the origin of life on earth. Among them, Rhodopseudomonas and Rhodospirillaceae are photosynthetic bacterial species with more industrial production. The protein content of photosynthetic bacteria is as high as more than 60%. At the same time, it is rich in coenzyme Q10 (ubiquinone) that can be used as a cell metabolism activator and natura...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 徐中文张金燕张兴锋周绍生张璐李朱萍孙建新刘硕许倩倩闫海
Owner 广东绿百多生物科技有限公司
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