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Culture medium suitable for preparing porcine circovirus type II vaccine through PK15 cell and using method thereof

A virus vaccine and culture medium technology, applied in the field of biological products, can solve the problems of difficult control of product batch differences, less research on culture medium, and difficult to clarify components, and achieve the effects of good cell shape, reduced risk, and fast growth rate.

Inactive Publication Date: 2013-11-27
JIANGSU TAIYI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, animal serum also has its obvious disadvantages: it is difficult to determine the composition, it is not easy to control the difference between product batches, it is expensive, and it requires extremely strict testing procedures to ensure that the serum does not contain bacteria, viruses, etc. that may be carried by the serum-derived animal
At present, there are few studies on the medium for preparing porcine circovirus type II vaccine by large-scale high-density cultivation of PK15 cells in bioreactors, and more in-depth research is still needed

Method used

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  • Culture medium suitable for preparing porcine circovirus type II vaccine through PK15 cell and using method thereof
  • Culture medium suitable for preparing porcine circovirus type II vaccine through PK15 cell and using method thereof
  • Culture medium suitable for preparing porcine circovirus type II vaccine through PK15 cell and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The medium of the PK15 cell large-scale high-density culture that uses, its composition and the ratio of parts by weight are as follows:

[0053]

[0054]

[0055] 2% fetal bovine serum was added to the above medium to prepare a medium for large-scale high-density culture of PK15 cells.

[0056] The composition of the medium used to maintain virus proliferation and the ratio of parts by weight are as follows:

[0057]

[0058]

[0059] On the basis of the above-mentioned medium, the following components are also added to make a culture solution for maintaining virus proliferation, and the ratio of its parts by weight is as follows:

[0060]

[0061]

[0062] The experimental results are shown in Table 1, the data of "Example 1" in Table 2.

Embodiment 2

[0064] The medium of the PK15 cell large-scale high-density culture that uses, its composition and the ratio of parts by weight are as follows:

[0065]

[0066]

[0067] 2% fetal bovine serum was added to the above medium to prepare a medium for large-scale high-density culture of PK15 cells.

[0068] The composition of the medium used to maintain virus proliferation and the ratio of parts by weight are as follows:

[0069]

[0070]

[0071] On the basis of the above-mentioned medium, the following components are also added to make a culture solution for maintaining virus proliferation, and the ratio of its parts by weight is as follows:

[0072]

[0073] The experimental results are shown in Table 1 and the data of "Example 2" in Table 2.

Embodiment 3

[0075] The medium of the PK15 cell large-scale high-density culture that uses, its composition and the ratio of parts by weight are as follows:

[0076]

[0077]

[0078] 2% fetal bovine serum was added to the above medium to prepare a medium for large-scale high-density culture of PK15 cells.

[0079] The composition of the medium used to maintain virus proliferation and the ratio of parts by weight are as follows:

[0080]

[0081]

[0082] On the basis of the above-mentioned medium, the following components are also added to make a culture solution for maintaining virus proliferation, and the ratio of its parts by weight is as follows:

[0083]

[0084] The experimental results are shown in Table 1, the data of "Example 3" in Table 2.

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Abstract

The invention provides a culture medium suitable for preparing a porcine circovirus type II vaccine by carrying out large-scale high-density culture on PK15 cells.

Description

technical field [0001] The invention belongs to the field of biological products, and relates to a method for preparing and using a medium, and more specifically, relates to a medium for preparing porcine circovirus type II vaccine by cultivating PK15 cells in a large-scale high-density growth environment. Background technique [0002] The preparation of final products through animal cell culture is one of the most commonly used production methods in the field of biological products. Most of the medium used for animal cell culture needs to add 10%-15% animal serum to provide the nutrients needed for cell growth and the supporting proteins for cells to maintain normal shape. However, animal serum also has its obvious disadvantages: it is difficult to determine the composition, it is difficult to control the difference between product batches, it is expensive, and it requires extremely strict testing procedures to ensure that the serum does not contain bacteria and viruses tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N7/02A61K39/12A61P31/20
Inventor 贾馨丹
Owner JIANGSU TAIYI BIOTECH
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