Method for establishing Bian chicken quick-grow type AA strain and slow-grow type GG strain colony
A slow-growing type and strain technology, which is applied in the field of establishing fast-growing AA strains and slow-growing GG strains of side chickens, can solve the problems of decreasing numbers, endangered varieties, and disorderly mating, etc., and achieves the effect of reasonable design.
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[0018] Specific embodiments of the present invention will be described in detail below.
[0019] A kind of method for setting up side chicken fast-growing type AA strain and slow-growing type GG strain population, comprises the steps:
[0020] (1) In the seventh-generation hemp-feather single-comb line chicken flock bred in closed-group families, 299 hen blood samples and 81 rooster blood samples were collected from the core flock of the seventh-generation side chicken. According to the previously established Bian chicken MSTN gene G2283A site detection method (PCR-RFLP), the MSTN genotype of the blood sample was checked, the PCR product was digested with BbvI, and the sample containing the 162bp target fragment was named Kuaichang Genotype AA; the sample containing the 127bp target fragment was named the slow-length genotype GG, and the AA and GG individuals in the hemp-feather single-comb line of Bian chicken were determined. Of the 299 blood samples from hens, 75 were AA, ...
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