Method for preparing non-human animal model with opportunistic infections and chronic wasting diseases and medicament screened by using same

A non-human animal, opportunistic technology, applied in anti-infective drugs, allergic diseases, metabolic diseases, etc., can solve the problems of adverse reactions, limited clinical efficacy, tissue and organ damage, etc., to reduce mortality, no toxic side effects, The effect of prolonging life

Inactive Publication Date: 2015-01-07
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) Congenital immunodeficiency
(3) Secondary immunodeficiency
In addition, the cause and formation mechanism of acquired immunodeficiency syndrome caused by non-HIV virus factors are still unclear, and its treatment is also an urgent problem to be solved
(5) Long-term application of certain antibiotics can inhibit the normal flora, which is conducive to the growth of certain conditional pathogens such as fungi, leading to opportunistic infections
Commonly used anti-infective drugs, such as antibacterial drugs, antifungal drugs, antiviral drugs, etc., but the application of anti-infective drugs will bring different degrees and types of adverse reactions, such as drug resistance, the double effect of long-term application of broad-spectrum antibacterial drugs "Immune Reconstitution Inflammatory Syndrome (IRIS)" after infection and AIDS antiretroviral therapy (ART) -- abnormal manifestations of worsening clinical symptoms and damage to important tissues and organs during the process of rebuilding the body's immune function
(2) Try to improve the immune function of the human body, such as strengthening nutrition, transfusing fresh blood or white blood cells, injecting γ-globulin, thymosin, specific immune ribonucleic acid, etc., but the clinical curative effect is limited and brings certain adverse reactions
The study of animal models of opportunistic infections and chronic wasting diseases with the characteristics of traditional Chinese medicine syndromes is blank, and the screening of opportunistic infections and chronic wasting diseases Therapeutic drugs and scientific and objective evaluation of curative effect have brought obvious obstacles

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Weighing: Aconite 150g, dried ginger 150g, licorice 150g.

[0045] The above raw materials are dry products.

[0046] Then,

[0047] (1) Broken to 1~3mm;

[0048] (2) Add 2500g of water, boil and extract on low heat for 4 hours;

[0049] (3) Remove residue by filtration to obtain 450ml of water extract. That is, each milliliter contains 1 gram of crude drug.

[0050] The obtained water extract is the traditional Chinese medicine compound for treating opportunistic infection and chronic wasting disease.

Embodiment 2

[0052] Weighing: Aconite 100g, dried ginger 100g, licorice 160g.

[0053] The above raw materials are dry products.

[0054] Then,

[0055] (1) Broken to 1~3mm;

[0056] (2) Add 3200g of water, boil and extract on low heat for 6 hours;

[0057] (3) Remove residue by filtration to obtain 450ml of water extract. That is, each milliliter contains 1 gram of crude drug.

[0058] The obtained water extract is the traditional Chinese medicine compound for treating opportunistic infection and chronic wasting disease.

Embodiment 3

[0060] Weighing: Aconite 180g, dried ginger 180g, licorice 100g.

[0061] The above raw materials are dry products.

[0062] Then,

[0063] (1) Broken to 1~3mm;

[0064] (2) Add 3220g of water, boil and extract on low heat for 6 hours;

[0065] (3) Remove residue by filtration to obtain 450ml of water extract. That is, each milliliter contains 1 gram of crude drug.

[0066] The obtained water extract is the traditional Chinese medicine compound for treating opportunistic infection and chronic wasting disease.

[0067] Modeling and Drug Therapy

[0068] Seventy-two Kunming male mice, weighing 16.9±2 g, aged 1 month, were provided by the Animal Experiment Center of Guilin Medical College, license number: GLMC20130308HL.

[0069] The 1-month-old clean-grade male mice were randomly divided into 2 groups: ① Normal control group (CTRL, 24 mice): 9:00-9:30, 12:30-13:00, 17:00-17 :30 Swimming in three time periods, giving 3ml of distilled water at 28°C after each swimming, for ...

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PUM

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Abstract

The invention discloses a method for establishing an animal model of opportunistic infection and chronic wasting disease, and also discloses a compound traditional Chinese medicine for treating opportunistic infection and chronic wasting disease, which consists of aconite 100 by weight ~180 parts, 100~180 parts of dried ginger, 100~160 parts of licorice. The positive effects of the present invention are: a method for establishing an animal model of opportunistic infection and chronic wasting disease has been found, and the model is highly controllable, reliable, repeatable, easy to use, and economical; the syndrome of the model conforms to clinical The pathogenesis of opportunistic infections and chronic consumptive diseases; the modeling factors conform to the theory of "Neijing", do not involve pathogenic organisms, reduce the risk of the experiment, and ensure biological safety; the traditional Chinese medicine compound obtained through screening has high efficiency, non-toxicity, wide range of drug sources, and is easy to use , economic and other characteristics, it has the function of prolonging the life of patients with opportunistic infections and chronic wasting diseases, improving the quality of life of patients, and improving the lesions of various tissues. No toxic and side effects have been seen in large doses.

Description

technical field [0001] The invention relates to a non-human animal model, in particular to a method for preparing a non-human animal model of opportunistic infection and chronic wasting disease. The invention also relates to a screening method using the model, and the screened drug for treating opportunistic infection and chronic wasting disease. Background technique [0002] Opportunistic infection refers to some pathogenic pathogens with weak pathogenicity, which cannot cause disease when the human immune function is normal, but when the human immune function is weakened, they take advantage of the weak point and invade the human body, causing various diseases. Normal flora can also cause infection under certain conditions such as low immune function of the body, changes in the site of residence, or imbalance of flora. It can be seen in the following situations: (1) The surface barrier function of the skin and mucous membrane is damaged, such as injury, burn, intubation, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02A61K49/00A61K36/9068A61P1/14A61P17/00A61P37/04A61P1/04A61P1/00A61P1/16A61P31/06A61P35/00A61P5/16A61P3/10A61P31/18A61P31/00
Inventor 赵海潞蒋莉萍梁荣感张均智戴支凯任亚萍莫刚
Owner GUILIN MEDICAL UNIVERSITY
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