Preparation method of C-type clostridium perfringens toxin vaccine of piglet red dysentery

Abstract

The invention relates to a preparation method of a C-type clostridium perfringens toxin vaccine of piglet red dysentery. The preparation method comprises the following steps of inoculating strains NCTC (National Collection Of Type Culture) 3180 of the C-type clostridium perfringens into a blood plate culture medium for carrying out anaerobic cultivation for 36 hours at 37 DEG C; carrying out shake cultivation on the culture by adopting a homemade toxin-producing medium (100 ml of PBS (Phosphate Buffer Solution), 2g of peptones, 1g of dextrin, 2g of yeast extracts, and 1.2g of L- arginine) under an anaerobic environment; inactivating the culture, filtering and adding an adjuvant to prepare the C-type clostridium perfringens toxin vaccine of the piglet red dysentery. During a using process, intramuscular injection is respectively adopted once a month and half a month before the delivery of a sow, wherein the dosage is 5-10 ml at a time. The C-type clostridium perfringens toxin vaccine of the piglet red dysentery is used for the sow at the later immunological gestation period, so that piglets obtain passive immunity through colostrums, and therefore, piglet red dysentery can be prevented. Moreover, the C-type clostridium perfringens toxin vaccine of the piglet red dysentery can be used for preventing sheep struck and calf enterotoksemia, so that the popularization of the C-type clostridium perfringens disease can be effectively controlled.

Description

(1) Field of invention [0001] The invention relates to a preparation method of a piglet red scour type C Clostridium perfringens toxoid vaccine. (2) Background of the invention [0002] Clostridium welchii, also known as Clostridium perfringens, can be divided into ABCDE five types according to the different toxins produced. Type C bacteria are mainly the pathogens of sudden attack in sheep. Newborn piglets are susceptible, manifested as bloody dysentery and high mortality, and can also cause enterotoxemia and necrotic enteritis in lambs, calves, and sheep. This bacteria mainly produces α, β two exotoxins. Since the mid-1980s, about 20 provinces and cities across the country first appeared sudden death syndrome in pigs, cattle, sheep and rabbits, which brought great losses to animal husbandry production. In recent years, the incidence of necrotizing enteritis and enterotoxemia in ruminants across the country is still on the rise, which has become a major problem that plagu...

AI Technical Summary

Problems solved by technology

In addition, there are triple inactivated vaccines for sheep rapid epidemic, sudden attack, and enterotoxemia, and inactivated vaccines for Clostridium perfringens type A in rabbits. These vaccines have a certain control effect on the epidemic of the disease, but there are also Some deficiencies, such as severe vaccination reactions of livestock, large injection doses of multiple vaccines, and inaccurate immune effects of some vaccines, the quality needs to be further improved