A complete set of equipment and detection method for on-site rapid detection of grain safety

A complete set of equipment and testing equipment technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that chemical analysis and instrument analysis methods cannot meet the requirements of rapid measurement and portability, and achieve saving nitrogen consumption, saving nitrogen blowing costs, and improving The effect of detection efficiency

Inactive Publication Date: 2016-05-18
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, chemical analysis and instrumental analysis methods cannot meet the requirements of rapid testing and portability, so there is an urgent need for a complete set of portable testing equipment, which can replace laboratory testing to a certain extent and achieve the effect of primary screening

Method used

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  • A complete set of equipment and detection method for on-site rapid detection of grain safety
  • A complete set of equipment and detection method for on-site rapid detection of grain safety
  • A complete set of equipment and detection method for on-site rapid detection of grain safety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Such as figure 1 As shown, a complete set of equipment for quickly detecting grain safety on the spot includes a detection box and detection equipment placed inside it. The detection box includes a box body 2 and a box cover 1 that is movably connected with it. Preferably, the The box body 2 and the box cover 1 are connected on one side and flexibly connected, and can be freely opened and fastened; the box body 2 and the box cover 1 can be fastened in various ways, such as setting zippers on the edges other than the connecting side , or set the locking mechanism to cooperate with each other to lock. The box body 2 has upper and lower layers, and the lower layer is a movable drawer 3. The drawer 3 is provided with a handle, which can be drawn and pushed freely. Tightening mechanism, after pushing in the lower drawer 3, fasten and lock the drawer 3. The locking mechanism is any structure that can achieve the purpose of locking, such as the double-control lock according ...

Embodiment 2

[0052] A method for detecting by using the complete set of equipment for rapidly detecting grain safety on site according to the present invention, comprising the steps of:

[0053] (1) Read the instruction manual thoroughly before performing the test, and return the test card and the sample to be tested to room temperature;

[0054] (2) Take out the colloidal gold rapid detection card from the original packaging bag;

[0055] (3) According to the properties of the sample, take a sample with a sample extraction tool and mark it with a marker pen; after crushing it with a pulverizer, weigh the sample on a micro balance, add it to a centrifuge tube, and add the sample extraction solution for extraction. The extraction tool includes a sample pulverizer , sample processing scissors, sample processing knife, sample spoon and micro balance;

[0056] (4) The sample extract is vortexed and centrifuged with a vortex centrifuge, and the supernatant is placed in a fully automatic liquid...

Embodiment 3

[0061] A method for detecting ochratoxin A in grains using the complete set of equipment for rapidly detecting grain safety on site according to the present invention comprises the following steps:

[0062] (1) Read the instruction manual thoroughly before performing the test, and return the test card and the sample to be tested to room temperature;

[0063] (2) Take out the ochratoxin A test card from the original packaging bag;

[0064] (3) Accurately weigh 2.5g of a representative uniformly pulverized sample into a centrifuge tube, then take 5mL of the sample extract, plug it tightly and shake for 5 minutes, let stand or centrifuge at 4000rpm for 5 minutes to obtain the supernatant;

[0065] (4) Take 1ml of the supernatant into a 15mL centrifuge tube, and evaporate to dryness at about 50°C with a fully automatic liquid level indicator nitrogen purging device;

[0066] (5) Add 500 μL of sample diluent to the centrifuge tube, vortex, and use a dropper to continuously suck an...

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Abstract

The invention discloses complete equipment for rapidly detecting grain cereal safety on site. The complete equipment comprises a detection box and detection equipment arranged inside the detection box, wherein the detection box comprises a double-layer box body and a box cover which is movably connected with the double-layer box body; the detection equipment comprises sample pretreatment equipment, rapid colloidal gold detection cards, an experiment reagent, reading and data processing equipment and experiment materials; the rapid colloidal gold detection cards comprise an aflatoxin B1 detection card, an ochratoxin A detection card, a zearalenone detection card and a vomitoxin detection card. According to the complete equipment, all equipment, reagents and materials required in safety detection on cereal feed are subjected to integrated innovation and are integrally combined, the effect of a mobile laboratory is achieved, and the complete equipment is applicable to on-site rapid detection on the grain cereal safety, so that the purpose that harmful substances can be discovered, monitored and managed in time is achieved; moreover, the complete equipment is convenient to carry and has the function of knock resistance and drop resistance.

Description

technical field [0001] The invention relates to a detection device for detecting the safety of grains and grains, in particular to a complete set of equipment capable of quickly detecting the safety of grains and grains on the spot, which integrates sample preprocessing equipment, detection reagents, detection cards, consumables, data reading and data processing The equipment is integrated, suitable for on-site rapid screening of grain safety. Background technique [0002] At present, mycotoxin contamination has become one of the important food safety issues. Mycotoxins widely pollute crops and their products. According to estimates by the Food and Agriculture Organization of the United Nations, about 25% of agricultural products in the world are polluted by mycotoxins every year, and 2% of grains lose their nutritional and economic value due to serious pollution, causing tens of billions of dollars in losses. Economic losses. The conventional mycotoxin analysis method has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
Inventor 邢仕歌储晓刚金涌熊齐荣钟强张悦
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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