Preparation method of barley extract fermented by lactobacillus and anti-tumor effect of barley extract fermented by lactobacillus

A technology of lactic acid bacteria fermentation and lactic acid bacteria, which is applied in the direction of anti-tumor drugs, food preparation, medical preparations containing active ingredients, etc., can solve the problems that anti-tumor extracts have not been found in patents or literature reports, etc., to achieve rich nutrition and functions, Ease of quality control and low production cost

Active Publication Date: 2013-12-18
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above-mentioned patents and literature reports on the anti-tumor research of barley products mainly use yeast fermentation, and the use of lactic acid bacteria to ferment barley to obtain anti-tumor extracts has not yet been seen in patents or literature reports.

Method used

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  • Preparation method of barley extract fermented by lactobacillus and anti-tumor effect of barley extract fermented by lactobacillus
  • Preparation method of barley extract fermented by lactobacillus and anti-tumor effect of barley extract fermented by lactobacillus
  • Preparation method of barley extract fermented by lactobacillus and anti-tumor effect of barley extract fermented by lactobacillus

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Experimental program
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Effect test

Embodiment 1

[0035] Weigh 20.0g of barley flour crushed through a 60-mesh sieve into a 500mL Erlenmeyer flask, add 1:6 ratio of water to it and mix evenly, first hydrolyze it with 0.015% β-glucanase at 40°C for 30 min, and then use 4 % Of Lactobacillus plantarum ( Lactobacillus plantarum Dy-1) Fermented at 30°C for 48h. After the fermentation is completed, FBE is centrifuged at 6000rpm / min for 20min at room temperature, the supernatant is collected, and freeze-dried for 24h to obtain FBE.

[0036] Weigh 0.5g of FBE dissolved in 50ml of deionized water, pass a 0.22um filter membrane, and adjust the filtrate to 0.125 mg / mL, 0.25 mg / mL, 0.5 mg / mL, 1.0 mg / mL, 2.0 mg / ml, 4.0 mg / ml was added to HT-29 tumor cells, and the cells were cultured for 24h, 48h, 72h, and the inhibition rate of FBE on colon cancer HT-29 cells was determined by MTT method. The results showed that the concentration of the extract was positively correlated with the inhibition rate of tumor cell proliferation, and the maximum...

Embodiment 2

[0038] The FBE prepared above was dissolved in physiological saline to prepare the concentrations of 0.2 g / ml and 0.1 g / ml, and was treated with a sterile membrane for use. Human HT-29 colon cancer cells were inoculated subcutaneously into the abdomen of BALB / c nude mice aged 4-6 weeks, and 2g / kg / d, 1g / kg / d, 2g / kg / d+5-FU ( 25 mg / kg / d, ip, 7 days) of FBE was administered to tumor-bearing nude mice by gavage for 30 consecutive days. At the same time, the same dose of normal saline group and intraperitoneal injection (25 mg / kg / d, 7 days) compared with the 5-FU group. The anti-tumor effect of FBE was confirmed by the volume of the transplanted tumor measured with a vernier caliper and the final tumor inhibition rate ( figure 2 ,3). The results showed that the tumor inhibition rate of the 2g / kg / d+5-FU mixed dose group could reach 53.36%.

[0039] Determination of superoxide dismutase (SOD) in the liver of tumor-bearing nude mice with a kit ( Figure 5 ), glutathione-peroxidase (GSH-...

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Abstract

The invention discloses a preparation method of a barley extract fermented by lactobacillus and an anti-tumor effect of the barley extract fermented by lactobacillus, belonging to the field of food biotechnology. Lactobacillus plantarum (lactobacillus plantarum Dy-1, CGMCC No.6016) is screened from pickled vegetables to serve as a fermentation strain for fermenting the barley, and fermentation conditions are controlled, so that the extract of fermented barley is obtained. Cell tests indicate that the extract has obvious effects on promoting apoptosis and suppressing multiplication of human colon cancer HT-29 cells and gastric cancer SGC-7901 cells, and a model test of the nude mouse bearing the tumor with the human colon cancer HT-29 indicates that the extract has obvious effects on suppressing tumor growth and improving the immune activity of the nude mouse bearing the tumor. According to the preparation method of the barley extract fermented by lactobacillus, the barley is pretreated simply and the production cost is low; and direct vat set fermentation is employed, so that the preparation method of the barley extract fermented by lactobacillus is convenient and rapid, safe and stable, and convenient to control the quality.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a fermented barley extract that uses barley as a raw material, is hydrolyzed by β-glucanase, and fermented by lactic acid bacteria to realize the biotransformation of nutrients, and to prepare a fermented barley extract capable of inhibiting tumor cell proliferation and tumor growth. The product of the invention (FBE) can be used in the preparation of functional food or medicine. Background technique [0002] At present, the number of deaths caused by malignant tumors in China accounts for 1 / 4 of the world's total, and the number is still rising. The treatment of cancer mainly includes surgery, chemotherapy, radiotherapy, and targeted therapy (immunotherapy, gene therapy, angiogenesis inhibitors, and targeted therapy), and the side effects still bring a lot of pain to patients. Therefore, it is the desire of cancer patients to develop anti-tumor active substances and functional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/105A23L1/29A61K36/8998A61P35/00A61K131/00A23L7/104A23L33/00
Inventor 董英肖香姚芳张家艳赵延胜徐斌
Owner JIANGSU UNIV
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