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Method for quickly detecting hyphantria cunea

An American white moth, rapid technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of difficult to make fast and accurate identification, difficult to distinguish, etc., to achieve good economic benefits and social effects, and operation Convenient and practical effect

Inactive Publication Date: 2013-12-18
SUZHOU CITY INVASIVE PEST PREVENTION & CONTROL TECH CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It is extremely difficult to distinguish the adult larvae of the American white moth from other species of the family Mothidae in morphology, especially when the eggs or incomplete worms of the American white moth are intercepted during port quarantine, it is more difficult to make a rapid and accurate identification by using traditional morphological classification

Method used

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  • Method for quickly detecting hyphantria cunea
  • Method for quickly detecting hyphantria cunea
  • Method for quickly detecting hyphantria cunea

Examples

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Embodiment 1

[0027] Using 16 species of Luphidae insects, including the American white moth, as test specimens, the adult larvae of these 16 species of Luphididae insects are indistinguishable from the American white moth in terms of external morphology. The test specimens are shown in Table 1.

[0028] Table 1 Test specimens

[0029]

[0030] Two pairs of specific primers COI01 / 02 and SpmR01 / 02 were designed for H. Subject to the state limitation of the tested insects, the adult larvae, pupae, eggs and remains of the American white moth can be accurately identified. The sequences of specific primers COI01 / 02 and SpmR01 / 02 are as follows:

[0031] COI01 sequence: 5'-CAGGAACTGGATGAACA-3',

[0032] COI02 sequence: 5'-CCTACTGCTCATACGAA-3',

[0033] SpmR01 sequence: 5'-ACGCTGTTGTCCGTATTT-3',

[0034] SpmR02 sequence: 5'-CTGATTTCTTCGGTGTTG-3'.

[0035] 1) DNA preparation: DNA was extracted using GenMagBio Animal Cell Tissue / Cell Genomic DNA Magnetic Beads Extraction Kit. The specific ...

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Abstract

The invention discloses a method for quickly detecting hyphantria cunea. The method comprises the following steps of preparing DNA, carrying out a PCR reaction system and reaction conditions, and judging PCR results. The method for quickly detecting the hyphantria cunea is an efficient, accurate and convenient hyphantria cunea molecule detection technology, and has the advantages of capability of distinguishing the hyphantria cunea and other species of arctiidae at a molecule level, capability of meeting the usage requirement and good practicability, so that good economic benefits and social effects can be generated.

Description

technical field [0001] The invention belongs to the technical field of white moth detection, and in particular relates to a method for rapidly detecting white moth. Background technique [0002] American white moth [Hyphantria cunea (Drury)], also known as autumn curtain caterpillar, autumn curtain moth, belongs to Lepidortera (Lepidortera), lamp moth family (Arctiidae), lamp moth subfamily (Arctiinae), is a world-renowned quarantine pest biology. Originated in North America, distributed in the United States, Canada, Mexico and other regions, it was introduced to Europe in 1940, Japan in 1945, South Korea in 1958, and Dandong, Liaoning, my country in 1979. At present, the domestically announced epidemic areas include Beijing, Tianjin, Hebei, Shandong, Liaoning, Shaanxi, Jiangsu and other provinces and cities. The General Office of the State Council has issued documents many times, requiring vigorous efforts to strengthen the prevention and control of white moths in the Uni...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 詹国辉陈景云郑斯竹高渊陈云芳邓海娟赵毓郎葛华林
Owner SUZHOU CITY INVASIVE PEST PREVENTION & CONTROL TECH CENT
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