Method for making epileptic seizure animal model

A technology of epileptic seizures and animal models, applied in animal husbandry and other fields, can solve the problems of rhesus monkey kainic acid-free epilepsy models, antiepileptic drugs can not control satisfactory seizures, etc., to achieve clear epilepsy process, small dose, drug safety good sex effect

Inactive Publication Date: 2014-01-15
MENG FAN'GANG
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

③ Antiepileptic drugs cannot control their satisfactory seizures
[0006] Primates are the closest relatives to humans, and there are still no reports on rhesus monkey kainate epilepsy models at home and abroad

Method used

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  • Method for making epileptic seizure animal model
  • Method for making epileptic seizure animal model
  • Method for making epileptic seizure animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] Establishment of rhesus monkey epilepsy model of the present invention

[0024] 1. Experimental materials

[0025] 1.1 Experimental animals

[0026] 5 healthy rhesus monkeys were purchased from the Institute of Animals, Chinese Academy of Medical Sciences. They were 3 years old and weighed 7.9kg, 7.8kg, 8.0kg, 8.1kg, and 8.2kg. They were fed 3 times a day, supplemented with vegetables and fruits, the average indoor temperature was (20±12.4)°C, and the relative humidity was 87.5±12.5. There was a 4-week adaptation period before the experiment, and neurological examinations and EEG examinations were performed to rule out outliers.

[0027] 1.2 Experimental drugs

[0028] Kainic acid: purchased from Sigma.

[0029] Anesthetics: Ketamine Hydrochloride Jiangsu Hengrui Medicine Co., Ltd. Specification 0.1g / 2ml

[0030] Sumianxin

[0031] atropine

[0032] 2. Main instruments and equipment

[0033] Zhongke Xintuo NT9200 series portable digital electroen...

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Abstract

The invention provides a method for making an epileptic seizure animal model. The method for making the epileptic seizure animal model comprises the step of applying kainic acid to primate animal sea horses by adopting a stereospecific technique. The made epileptic seizure animal model has the advantages of local injection administration, low dosage, little relation between epileptogenic dosage and genders and ages of animals, good repeatability, clear epileptogenic process and good drug safety.

Description

technical field [0001] The invention relates to a method for preparing an epileptic seizure animal model and the epileptic seizure animal model prepared by the method. Specifically, the present invention relates to a method for preparing an animal model of epileptic seizures by microinjecting kainic acid into the hippocampus of a primate, and the epileptic seizure model of a primate prepared by this method. Background technique [0002] Seizures are epileptic brain function abnormalities caused by abnormal discharge of brain neurons. Most of the seizures are short-lived and self-limited. Due to the different brain functional areas of the abnormal discharge, there may be a variety of seizure manifestations clinically, including partial seizures or generalized seizures. Neurological dysfunction. Generalized seizures and complex partial seizures are often accompanied by varying degrees of disturbance of consciousness. [0003] Epilepsy is a transitional synchronous abnormal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/401A01K67/027
Inventor 孟凡刚张建国陈宁杨岸超刘焕光葛燕刘崇胡文瀚张凯
Owner MENG FAN'GANG
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