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Method for separating and purifying kainic acid through controllable multichannel electroosmosis

An electrodialysis and multi-channel technology, applied in electrodialysis, organic chemistry, etc., can solve the problems of high price, difficulty in ensuring activity, and high cost, and achieve the effects of increasing speed, realizing separation, and increasing yield

Inactive Publication Date: 2012-10-31
YANTAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organic synthesis requires about 20 steps, the yield is very low, and the activity is difficult to guarantee, and the cost is also high
The current seaweed extraction process is extraction and ion exchange method, and its yield is very low, so the price of KA remains high

Method used

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  • Method for separating and purifying kainic acid through controllable multichannel electroosmosis
  • Method for separating and purifying kainic acid through controllable multichannel electroosmosis

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: A method for separating and purifying kainic acid by controllable multi-channel electrodialysis, comprising the steps of:

[0028] A. Collect partridge or sea algae, dry and crush into seaweed particles;

[0029] B, the seaweed particles are reflux cooked twice with 60% ethanol with a mass concentration, and the filtrate is combined; the mass ratio of ethanol to seaweed particles is 2:1

[0030] C, the filtrate is filtered with ultrafiltration to remove particulate impurities;

[0031] D. Use controllable multi-channel electrodialysis to remove salts, polysaccharide compounds and other impurities in the filtrate;

[0032] E. Use an ion exchange resin column for further separation and purification to remove impurities similar in nature to kainic acid;

[0033] F. Vacuum drying and crystallization to obtain kainic acid.

[0034] Controllable multi-channel electrodialysis consists of a plate, a separator, an anion exchange membrane, and a cation exchange me...

Embodiment 2

[0035] Example 2: The method for separating and purifying kainic acid with controllable multi-channel electrodialysis is different from the example in that in step B, the seaweed particles are refluxed and cooked 4 times with ethanol with a mass concentration of 80%, and the filtrate is combined; the ethanol and seaweed particles Mass ratio is 4:1, and others are with embodiment 1. The difference from Example 1 is that the controllable multi-channel electrodialysis channel ① enters the unpurified stock solution containing kainic acid, the pH value is controlled at 2.5, and the channel ② enters NaNO 3 Solution, pH 7, channel ③ into NaNO 3 solution, pH 7. Adjust the pH value of the channel by adding hydrochloric acid or NaOH solution, and add it while measuring the pH value until the pH reaches the requirement.

Embodiment 3

[0036] Example 3: Method for separating and purifying kainic acid by controllable multi-channel electrodialysis: figure 2 It is a schematic diagram of the structure of controllable multi-channel electrodialysis. Controllable multi-channel electrodialysis is composed of plate, separator, anion exchange membrane and cation exchange membrane. Chamber ④ and cathode chamber ⑤, channel ① enters unpurified stock solution containing kainic acid, the pH value is controlled at 2.0, channel ② enters NaNO 3 solution, pH 6.5, channel ③ into NaNO 3 solution, pH 6.5. Channel ① is filled with cation exchange resin (001x7, purchased from Nankai University Chemical Plant), channels ② and ③ are added with mixed bed ion exchange resin (anion exchange resin is 201x7, purchased from Nankai University Chemical Plant), and the mass ratio of anion and cation exchange resins is 1:1. figure 1 It is a process flow diagram of the present invention. It can be seen from this figure that the partridge ...

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Abstract

The invention provides a method for separating and purifying kainic acid through controllable multichannel electroosmosis, which comprises the following steps of: drying Caloglossa leprieurii or digenea, and crushing into small particles; refluxing and cooking the particles by using ethanol, and mixing filtrate; performing ultrafiltration on the filtrate to remove particle impurities; removing salts, polysaccharide compounds and other impurities from the filtrate through controllable multichannel electroosmosis; further separating and purifying by using an ion exchange resin column to remove impurities such as amino acid with similar properties to the kainic acid; and performing vacuum drying and crystallization to obtain the kainic acid.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and in particular relates to a process and a device for extracting kainate by multi-channel controllable electrodialysis. Background technique [0002] Kainic acid (KA) is an agonist of non-NMDA glutamate receptors. A large number of experiments have proved that the activation of non-NMDA receptors is an important link in many acute and chronic nervous system degenerations. In 1976, after Coyle and Schwarcz injected KA into the striatum of rats, they found that the GABAergic and acetylcholinergic neurons in the striatum of the rats were lost to varying degrees, and the rats also showed rotation to the opposite side of the injury. Behavioral changes (Coyle and Schwarcz, 1976). The main pathological changes of Huntington's disease are similar to this, so intrastriatal injection of KA in rats is considered to simulate Huntington's disease and is used to study the protective effect of dif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D207/16B01D61/42
Inventor 韩晓丽冯咏梅赵旗满杰王文华任万忠
Owner YANTAI UNIV
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