Preparation method for microecologics for laying hen feeds and application of microecologics
A technology of probiotics and laying hens, applied in application, animal feed, animal feed, etc., can solve problems such as no better solution, achieve enhanced anti-stress ability, improve activity and stability, and increase pH in the stomach value effect
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Embodiment 1
[0035] A preparation method for probiotics for laying hens, comprising the following steps:
[0036] (1) The strains of Lactobacillus plantarum and Bacillus subtilis are enriched and cultured in a fermenter respectively and then mixed to obtain a mixed bacterial liquid;
[0037] (2) Fully mix the mixed bacterial liquid with the aqueous solution containing pectin, Tween 80, glycerin, mannitol, trehalose and sodium bicarbonate according to the same volume, and make microencapsulated live bacterial powder after spray drying (See Figure 9 ); the mass percentages of pectin, Tween 80, glycerin, mannitol, trehalose, and sodium bicarbonate in the aqueous solution are respectively 0.5-1%, 0.8-1.5%, 0.8-1.2%, 0.5-1%, 3 -6%, 2-3.5%;
[0038] (3) Uniformly mixing the microencapsulated live bacteria powder with selenized carrageenan, zinc methionine and auxiliary materials to prepare a microecological preparation for laying hens.
[0039] Each gram of the probiotics for laying hens con...
Embodiment 2
[0046] After mixing the liquid fermented product of Lactobacillus plantarum and Bacillus subtilis cultured in the fermenter in proportion, with 0.5% pectin, 0.8% Tween 80, 0.8% glycerin, 0.5% mannitol, 6% trehalose and 3.5 After the solution of % sodium bicarbonate was fully mixed according to the same volume, it was spray-dried to make live bacteria powder, and then the bacteria powder was evenly mixed with selenized carrageenan, zinc methionine and glucose to obtain a microecological preparation for laying hens. After testing, it was shown that each gram of probiotics contained 250 million Lactobacillus plantarum, 1 billion Bacillus subtilis, and the contents of selenized carrageenan and zinc methionine were 0.5% and 3% respectively. Add the prepared probiotics into the basal ration at a ratio of 1%, and feed it to laying hens after thorough stirring.
[0047] The experiment selected two cage-raised laying hens, each with 10,000 chickens, and divided them into a control grou...
Embodiment 3
[0065] After mixing the liquid fermented product of Lactobacillus plantarum and Bacillus subtilis cultured in the fermenter in proportion, with 1% pectin, 1.5% Tween 80, 1.2% glycerin, 1% mannitol, 3% trehalose and 2 After the aqueous solution of sodium bicarbonate is fully mixed by the same volume, live bacteria powder is made after spray drying, and then the bacteria powder is mixed with selenized carrageenan, zinc methionine and glucose. It contains 500 million Lactobacillus plantarum, 2.5 billion Bacillus subtilis, 1.2% selenized carrageenan and 1.5% zinc methionine. The prepared probiotics were added to the basal diet at a ratio of 1%, and fed to laying hens after thorough stirring.
[0066] After tissue and cell damage, in addition to activating neutrophils and macrophages to cause the release of products such as arachidonic acid and prostaglandins and the production of oxygen free radicals, it can also stimulate monocytes to release TNF-a and other inflammatory cells f...
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