Biological substrate modification composite microbial agent and preparation method thereof

A compound microbial inoculum and a technology of compound microorganisms are applied in the field of biological bottom modification compound microbial inoculum and its production, which can solve the problems of thin water quality, weakened self-purification ability, harm to water bodies and breeding animals, etc., so as to promote growth and improve microecology. Balance and inhibit the growth of pathogenic bacteria

Pending Publication Date: 2022-04-12
无锡三智生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the reasonable application of physical and chemical bottom reform can quickly improve the transparency of water body, prevent the water quality from being too thick, reduce the content of ammonia nitrogen and nitrite at the bottom, promote feeding, and inhibit the proliferation of pathogenic bacteria to a certain extent, but long-term or even high-dose use will have negative effects on the water quality. Water bodies and farmed animals cause considerable harm. For example, excessive use of adsorption and flocculation substrates can easily cause water quality to be too thin, water transparency is too high, and self-purification ability is weakened. Excessive use of oxidative substrates will cause stress reactions in farmed animals, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, a kind of bio-refined composite microbial agent, including Bacillus subtilis, nitrifying bacteria, denitrifying bacteria, sulfurizing bacteria, and auxiliary materials.

[0034] In this example, the mixing mass ratio of the composite microbial agent is: Bacillus subtilis: nitrifying bacteria: denitrifying bacteria: sulfurizing bacteria: auxiliary materials = 3:2:1:1:0.2.

[0035] In this example, the auxiliary material is a mixture of potassium fulvic acid, aluminum silicate and magnesium sulfate.

[0036] In this example, the mixing mass ratio of the auxiliary materials is: potassium fulvic acid, aluminum silicate, and magnesium sulfate=1:1:1.

[0037] In this example, Bacillus subtilis selects a low-temperature Bacillus subtilis bacterial liquid that can maintain activity above 8°C.

[0038] In this example, the live bacteria content of the bacillus subtilis is 2×10 8 Individual / g, the nitrifying bacteria live bacteria content is 2×10 8 Each / g, the live...

Embodiment 2

[0046] Embodiment 2, a kind of bio-refined composite microbial agent, including Bacillus subtilis, nitrifying bacteria, denitrifying bacteria, sulfurizing bacteria, and auxiliary materials.

[0047] In this example, the mixing mass ratio of the composite microbial agent is: Bacillus subtilis: nitrifying bacteria: denitrifying bacteria: sulfurizing bacteria: auxiliary materials = 3:2:1:1:0.2.

[0048] In this example, the auxiliary material is a mixture of potassium fulvic acid, aluminum silicate and magnesium sulfate.

[0049] In this example, the mixing mass ratio of the auxiliary materials is: potassium fulvic acid, aluminum silicate, and magnesium sulfate=1:1:1.

[0050] In this example, Bacillus subtilis selects a low-temperature Bacillus subtilis bacterial liquid that can maintain activity above 8°C.

[0051] In this example, the live bacteria content of the bacillus subtilis is 2×10 10 Individual / g, the nitrifying bacteria live bacteria content is 2×10 10 Each / g, the ...

Embodiment 3

[0059] Embodiment 3, a kind of bio-refined composite microbial agent, including Bacillus subtilis, nitrifying bacteria, denitrifying bacteria, sulfurizing bacteria, and auxiliary materials.

[0060] In this example, the mixing mass ratio of the composite microbial agent is: Bacillus subtilis: nitrifying bacteria: denitrifying bacteria: sulfurizing bacteria: auxiliary materials = 3:2:1:1:0.2.

[0061] In this example, the auxiliary material is a mixture of potassium fulvic acid, aluminum silicate and magnesium sulfate.

[0062] In this example, the mixing mass ratio of the auxiliary materials is: potassium fulvic acid, aluminum silicate, and magnesium sulfate=1:1:1.

[0063] In this example, Bacillus subtilis selects a low-temperature Bacillus subtilis bacterial liquid that can maintain activity above 8°C.

[0064] In this example, the live bacteria content of the bacillus subtilis is 2×10 9 Individual / g, the nitrifying bacteria live bacteria content is 2×10 9 Each / g, the li...

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PUM

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Abstract

The invention discloses a biological bottom modification compound microbial agent and a preparation method thereof, and belongs to the field of water plant cultivation, and the biological bottom modification compound microbial agent comprises bacillus, nitrifying bacteria, thiobacteria and photosynthetic bacteria; the biological bottom modification is a brand-new bottom material improvement concept, takes a pond culture water area as an independent ecological system, and aims to keep the stability of the pond ecological system through a bacterial phase and an algal phase. According to the bottom modification, dominant populations are formed at the bottom mainly through growth and reproduction of beneficial microorganisms, organic matters at the bottom are strongly decomposed and converted into inorganic nutrient substances such as carbon dioxide, nitrate and sulfate, the inorganic nutrient substances are absorbed and grow by algae and aquatic plants, the bottom environment is improved, the living space of harmful bacteria is squeezed, and breeding of pathogenic bacteria is inhibited. Beneficial microorganisms can improve in-vivo micro-ecological balance of bred animals and improve the health level, and meanwhile, as high-quality single-cell protein, the beneficial microorganisms can promote growth of the bred animals and increase yield and efficiency.

Description

technical field [0001] The application belongs to the technical field of aquaculture, and in particular relates to a biologically modified composite microbial agent and a preparation method thereof. Background technique [0002] The bottom quality refers to the bottom environment of the pond, including bottom mud and bottom water quality. Due to the low density of aquatic animals and the natural flow of water in natural waters, it will not cause excessive accumulation of nutrients at the bottom, and the thickness of bottom mud and bottom water quality will be stable. state, the bacteria and algae are relatively balanced, and aquatic animal diseases rarely occur. [0003] Intensive aquaculture is to make full use of the limited aquaculture water body, high-density aquaculture, and high feed feeding volume to achieve the goal of high yield. The negative effects brought by it are all-round and obvious-severe eutrophication of aquaculture water body, blue-green algae Frequent o...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F11/02C02F3/34C12R1/125C12R1/01
Inventor 蒋蓉曹建民王敏
Owner 无锡三智生物科技有限公司
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