Application of cinnamon extract to preparation of medicines or healthcare products for treating or preventing tumour

A technology of plant extracts and health products, applied in the application field of medicine or health products, can solve problems such as environmental pollution, increased incidence of malignant tumors, and threats to human health

Active Publication Date: 2014-01-22
临沂山松药业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the development of society, the improvement of human living standards and medical environment, the life expectancy of human beings has been significantly extended, but at the same time of rapid economic development, serious environmental pollution has led to an increase in the incidence of malignant tumors, which seriously threatens human health. heavy burden on the state and society

Method used

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  • Application of cinnamon extract to preparation of medicines or healthcare products for treating or preventing tumour
  • Application of cinnamon extract to preparation of medicines or healthcare products for treating or preventing tumour
  • Application of cinnamon extract to preparation of medicines or healthcare products for treating or preventing tumour

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Activation of Cinnamon Plant Extracts on Nrf2 Signaling Pathway

[0027] Methods: (1) Preparation of Cinnamomum plant extracts

[0028] Plants of the genus Cinnamon (respectively: cinnamon, camphor, camphor, camphor, yin xiang, and sassafras, a total of 6 kinds of camphor plant extracts were prepared) aerial part 200g, crushed, 75% ethanol 800mL, extracted twice , 1 hour each time, the extracts were combined, concentrated and dried to obtain 6 camphor plant extracts for activity evaluation.

[0029] (2) Culture of human breast cancer cell line MDA-MB-231

[0030] Human breast cancer cell line MDA-MB-231 was purchased from the American Type Culture Collection (ATCC), using MEM medium containing 10% fetal bovine serum (FBS), 2 mM HEPES and 6 ng / mL insulin, placed at 37 ° C, 5 %CO2 cultured in an incubator.

[0031] (3) ARE-dependent luciferase reporter assay

[0032] MDA-MB-231-Luc cells were seeded on a 96-well plate, and after 24 hours, different concentr...

Embodiment 2

[0034] Example 2: Cinnamomum plant extracts can up-regulate the expression of Nrf2 and its downstream gene protein levels

[0035] Method: Western blot analysis (Western blot) to detect changes in protein levels in cells

[0036] MDA-MB-231 cells were inoculated in a 35 mm diameter petri dish, cultured until the density reached 70% to 80%, and then treated with different concentrations of Cinnamomum plant extracts (Cinnamomum genus plant extracts were prepared in Example 1) for 16 hours, Wash twice with PBS, add cell lysate (50μg / ml aprotinin, 0.5mM phenylmethylsulfonyl fluoride, 1mM sodium orthovanadate, 10mM sodium fluoride, 10mM β-glycerol phosphate), collect protein and measure it by Bradford method protein concentration. Each sample protein (100 μg) was loaded on the sample, and the protein components were separated by SDS-PAGE, and the protein bands were transferred to the nitrocellulose membrane by electrotransfer method. After the film was sealed with 5% skimmed milk...

Embodiment 3

[0038] Example 3: Cinnamon Plant Extract Increases Intracellular Glutathione Levels

[0039] Methods: (1) Culture of human normal lung epithelial cells HBE cells

[0040] Human normal lung epithelial cells HBE were purchased from the American Type Culture Collection (ATCC), using MEM medium, and adding 10% fetal bovine serum (FBS), 5% glutamine, placed at 37 ° C, 5% CO 2 cultured in an incubator.

[0041] (2) Determination of intracellular glutathione content

[0042] Inoculate HBE cells in a 35mm diameter petri dish, culture until the density reaches 70% to 80%, add different concentrations of Cinnamon plant extracts (Cinnamomum camphora plant extracts are prepared in Example 1) for 24 hours, wash with PBS for 2 Once, add 0.5mL of 50mM sodium phosphate and 1mM EDTA buffer to collect the cells, sonicate for 1min, centrifuge at 10000g for 15min, take the supernatant, operate according to the instructions of the glutathione assay kit, measure the absorbance at 412nm and calcu...

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Abstract

The invention discloses novel application of a cinnamon extract, which relates to application of cinnamon (including cinnamon, chartophyllum, cinnamomum mollifolium, howood, cinnamomum burmannii and sassafras) extract to preparation of medicines or healthcare products for treating or preventing tumour. The invention also provides a preparation method of the cinnamon extract. Researches show that the cinnamon extract is capable of activating an Nrf2 signal path, promoting anti-oxidation and expression of II phase detoxifying enzymes, increasing the intracellular glutathione level, enhancing the intracellular reduction capability and inhibiting the cytotoxicity caused by cancerogen arsenic and reactive oxygen H2O2. Consequently, the cinnamon extract can be prepared into corresponding oral preparations and injections and used for preventing the tumour.

Description

technical field [0001] The present invention relates to a new application of the plant extract of the genus Cinnamomum, in particular to the application of the plant extract of the genus Cinnamomum in the preparation of medicines or health care products for treating or preventing tumors. Background technique [0002] With the development of society, the improvement of human living standards and medical environment, the life expectancy of human beings has been significantly extended, but at the same time of rapid economic development, serious environmental pollution has led to an increase in the incidence of malignant tumors, which seriously threatens human health. The state and society have brought a heavy burden. On the premise that there is no effective drug to achieve effective treatment of malignant tumors, the development of preventive drugs and health products to prevent the early formation and occurrence of diseases has become an effective coping strategy. [0003] T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/54A61K9/48A61K9/20A61K9/14A61K9/16A61K9/08A61P35/00A23L1/30A23L33/105
Inventor 沈涛李国辉任冬梅
Owner 临沂山松药业有限公司
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