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Wheat heat stress associated protein TaGCN5 as well as coding gene and application thereof

A gene and protein technology is applied to the wheat heat stress related protein TaGCN5 and its encoding gene and application field, which can solve the problems of grain yield decrease, quality deterioration, influence on grain filling, protein and starch synthesis, etc., to increase the ability of high temperature stress tolerance Effect

Active Publication Date: 2014-01-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High temperature has a very important impact on the growth and development of wheat. High temperature will not only affect the development, flowering and fertilization of ovary, ovule sac and pollen, but also affect the synthesis of grain filling, protein and starch, resulting in a decrease in grain yield and poor quality.

Method used

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  • Wheat heat stress associated protein TaGCN5 as well as coding gene and application thereof
  • Wheat heat stress associated protein TaGCN5 as well as coding gene and application thereof
  • Wheat heat stress associated protein TaGCN5 as well as coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Discovery of wheat heat stress-related protein TaGCN5 and its encoding gene

[0035] On the basis of the NCBI database, the genes and fragments with related functions from other plants were compared, and according to the results of comparison and sequence analysis, the relevant fragments of wheat were spliced, extended and functionally identified, and finally a The completed open reading frame encodes the protein shown in Sequence 1 of the Sequence Listing, and the protein is named TaGCN5 protein. The gene encoding TaGCN5 protein is named as TaGCN5 gene, as shown in sequence 2 of the sequence listing.

Embodiment 2

[0036] Embodiment 2, the acquisition and identification of transgenic plants

[0037] 1. Construction of recombinant plasmids

[0038] 1. Synthesize the double-stranded DNA molecule shown in sequence 2 of the sequence listing.

[0039] 2. Using the double-stranded DNA molecule synthesized in step 1 as a template, perform PCR amplification with a primer pair composed of F1 and R1 to obtain a PCR amplification product.

[0040] F1: 5'-CACCCGG GGTACC ATGGACGGCCTCGCGGCGCC-3';

[0041]R1: 5'-CTGGGTC GAGCTC CTAGTTCTTCGTTGACGCAG-3'.

[0042] 3. The PCR amplified product of step 2 was double-digested with restriction endonucleases KpnI and SacI, and the digested product was recovered.

[0043] 4. Digest the pCAMBIA-1300 vector with restriction endonucleases KpnI and SacI to recover the vector backbone.

[0044] 5. Ligate the digested product of step 3 with the vector backbone of step 4 to obtain a recombinant plasmid. According to the sequencing results, the structure of the ...

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Abstract

The invention discloses a wheat heat stress associated protein TaGCN5 as well as a coding gene and an application thereof. The protein is named as protein TaGCN5 and is the following (a) or (b), namely, (a) a protein formed by amino acid sequences shown in a sequence 1 in a sequence table; and (b) a protein obtained through substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequences in the sequence 1, associated with high temperature stress resistance of plants and is derived from the sequence 1. An over-expression vector of a gene of TaGCN5 is constructed and the high temperature stress resistance of plants is obviously improved after transforming arabidopsis thaliana. The protein TaGCN5 has a great value in culturing high temperature resistant plants.

Description

technical field [0001] The invention relates to a wheat heat stress-related protein TaGCN5, its coding gene and application. Background technique [0002] During the growth and development of crops, in addition to being attacked by biological factors such as diseases and insect pests, they are also often affected by adverse climate and soil factors, which affect the yield and quality. This adverse effect is called environmental stress or abiotic stress. . With global warming, water shortage, soil desertification and salinization, the abiotic stress experienced by crops will become more frequent and more serious. [0003] Wheat is an important food crop in my country, and its yield and quality are directly related to people's lives. High temperature has a very important impact on the growth and development of wheat. High temperature will not only affect the development, flowering and fertilization of ovary, ovule sac and pollen, but also affect the synthesis of grain fillin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84C12N1/21C12N15/11A01H5/00
CPCC07K14/415C12N15/8271
Inventor 孙其信胡兆荣宋娜倪中福姚颖垠彭惠茹解超杰杜金昆梁荣奇
Owner CHINA AGRI UNIV