Biopesticide containing natural fungus and preventing and treating monochamus alternatus in high-efficiency manner and application
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A kind of natural fungus, the technology of Longmoe longicornis
Active Publication Date: 2014-01-29
JIYANG COLLEGE OF ZHEJIANG A & F UNIV
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But in any case, due to the uneven generation of longhorn beetles, the cost of chemical control is high and i
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Embodiment 1
[0017] In 50ml corn juice and 50ml sterile water, add 0.8g beef extract, 2.7g sucrose, 7g sodium chloride, 0.8g peptone, 0.7g dipotassium hydrogen phosphate, 0.8g magnesium sulfate, stir and dissolve to make a nutrient solution; Then add 3g Beauveria bassiana oosporium, 3g Verticillium mould, 3g Paecilomyces, 1g Branchomyces, 5g Penicillium, 3g Fusarium mould, 3g Trichoderma and 4g saccharomyces in the reaction kettle, then add 71g nutrient solution , 4g of sodium alginate, mixed evenly at a constant temperature of 37°C, and finally stored at 25°C.
Embodiment 2
[0019] In 50ml corn juice and 50ml sterile water, add 0.5g beef extract, 1.6g sucrose, 6g sodium chloride, 0.6g peptone, 0.5g dipotassium hydrogen phosphate, 0.6g magnesium sulfate, stir and dissolve to make a nutrient solution; Then add 2g Beauveria bassiana, 5g Beauveria bassiana, 5g Paecilomyces, 5g Branchomyces, 5g Penicillium, 5g Fusarium, 3g Trichoderma and 2g saccharomyces in the reaction kettle, then add 58g Nutrient solution, 5g sodium alginate and 5g Tween-80, mixed evenly at a constant temperature of 25°C, and finally stored at 25°C.
Embodiment 3
[0021] In 50ml corn juice and 50ml sterile water, add 0.4g beef extract, 2.5g sucrose, 5g sodium chloride, 0.5g peptone, 0.4g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, stir and dissolve evenly to make a nutrient solution; Then add 6g of Beauveria bassiana, 3g of Beauveria bassiana, 3g of Beauveria oosporium, 3g of Verticillium, 3g of Paecilomyces, 2g of Branchomyces, 2g of Penicillium, 2g of Fusarium in the reaction kettle , 1g of Trichoderma and 1g of yeast, then add 69g of nutrient solution, 5g of Tween-80, mix evenly at a constant temperature of 27°C, and finally store at 25°C.
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Abstract
The invention discloses biopesticide containing natural fungus and preventing and treating monochamus alternatus in a high-efficiency manner and application and belongs to the technical field of biopesticide. The biopesticide is characterized by comprising the following substances in percentage by weight: 20-40% of natural fungus, 50-76% of nutrient solution and 2-15% of a stabilizing agent. The biopesticide and the application have the advantages that the natural fungus for preventing and treating the monochamus alternates is adopted as the material, and is dispersed in nutrient solution which can enable the fungus to retain the activity for long time, then a certain amount of stabilizing agent is added to ensure the stability of the preparation, and the prepared preparation has good effect in preventing and treating the monochamus alternatus, so that the prevention and the treatment to pine wood nematode can be achieved by controlling the monochamus alternatus, and the occurrence of the pine wood nematode disease can be reduced; and the processing method is simple and convenient, the materials are sufficient, the storage and the transportation are safe, the cost is low and no environmental hazard is caused.
Description
technical field [0001] The invention belongs to the technical field of biopesticides, in particular to a biopesticide containing natural fungi for efficient control of Monochamus alternatus and its use. Background technique [0002] Monochamus monochamus is the main vector for the transmission of pine xylophilus. Japan has observed and studied Monochamus alternatus in many places since the 1960s, and has done a lot of research work on its biological characteristics, occurrence rules, the relationship between Monochamus alternata and B. xylophilus, and its control. In 1987, Tomio Kobayashi reported that Monochamus monochamus had one annual generation in Japan, and a few biennial ones in colder regions. Adults emerge from May to July, and mid-June is the eclosion period. After the eclosion adults drill out of the eclosion hole, they need to supplement nutrition for 2-3 weeks, eat the cortex of twigs less than three years old, and then mate and lay eggs. Nishizawa Kobayashi e...
Claims
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