Application of atractylodes macrocephalaon polysaccharide in medicaments or healthcare products for improving kuffer cell immune function
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A technology of Atractylodes macrocephala polysaccharide and cellular immunity, applied in the field of traditional Chinese medicine
Inactive Publication Date: 2014-02-05
SHAANXI INST FOR FOOD & DRUG CONTROL
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Embodiment 1
[0019] Example 1 Homemade Atractylodes Polysaccharides (PAM)
[0020] DEAE-cellulose filler was purchased from Beijing Ruida Henghui Technology Development Co., Ltd.
[0021] Take about 1000g of Atractylodes macrocephala, crush it into coarse particles, soak for 30min, heat and reflux with 95% ethanol to degrease for 2h, extract the filter residue three times with 3 times the amount of water, combine the water extracts, and concentrate. The concentrated solution was repeatedly washed three times with ethanol and acetone, and the precipitate was freeze-dried to obtain the crude polysaccharide of Atractylodes macrocephala. Atractylodes rhizome crude polysaccharides were dissolved in a small amount of distilled water, separated on a DEAE-cellulose column, and eluted with distilled water and 0.1mol / L NaCl solution respectively. Polysaccharides were partially eluted with distilled water. Dialyze the eluent, concentrate the dialysate, add ethanol to a concentration of 75% (V / V), l...
Embodiment 2
[0025] Embodiment 2 mouse experiment
[0026] 1. Instruments and materials
[0027] Experimental Animals Healthy BALB / C mice weighing 20-30 g, both male and female, were provided by the Animal Experiment Center of Xi'an Jiaotong University School of Medicine. In an environment with a constant temperature and a photoperiod of 12h:12h, the animals were fed for 1wk and then used for the experiment. Before the experiment, they were fasted for 24h and allowed to drink water.
[0028] Drugs and treatment Atractylodes macrocephala polysaccharide (PAM, self-made, purity greater than 99.9%), extracted from the dried root of Atractylodes macrocephala (identified as Atractylodes Macrocephala Koidz. by Professor Guo Zengjun, Department of Pharmacognosy, School of Medicine, Xi'an Jiaotong University), soluble in water, the main component For arabinose, mannose, glucose, galactose. Dissolve in distilled water before use, sterilize by autoclaving, and take the supernatant after centrifugat...
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Abstract
The invention relates to application of atractylodes macrocephalaon polysaccharide in medicaments or healthcare products for improving kuffer cell immune function, belonging to the technical field of traditional Chinese medicine. According to the application, homemade PAM (atractylodes macrocephalaon polysaccharide) is adopted for testing the influences on the mouse Kupffer cell immune function. The PAM in vitro is acted to a normal Kupffer cell so as to evaluate the immune function and the cell damage situation according to the phagocytosis quantity of neutral red A540, the secreting quantity of NO and TNF (tumor necrosis factor)-alpha as well as the LDH (lactate dehydrogenase) leakage quantity; after in-vivo mice are injected with the PAM, the indexes of the Kupffer cell, the activity of intracellular ACP (acid phosphatase) as well as the serum ALT and GST are detected. The result shows that the PAM intensifies the phagocytosis to the neutral red caused by Kupffer cell, improves the ACP activity as well as increases the generation amount of NO and TNF-alpha. The PAM in vitro does not increase parenchymal hepatic cell LDH leakage, and has no influence on the activities of sALT and sGST, so that the PAM can activate the Kupffer cell immune function, improves the organism disease resistance, and has an actual application value at the treatment aspect of diseases of immunodeficiency disease, tumor and the like.
Description
technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to the application of Atractylodes macrocephala polysaccharides extracted from traditional Chinese medicines in medicines or health care products for improving the immune function of kuffer cells. Background technique [0002] There are a wide range of immune active polysaccharides in medicinal plants, which have various functions such as inhibiting tumor growth, activating immune cells, and improving the immune function of the body. Atractylodes macrocephala is the dried rhizome of Atractylodes macrophala Koedz, a plant of Compositae, and is one of the famous "eight flavors of Zhejiang". Atractylodes macrocephala is the most commonly used tonic Chinese medicine, known as "Southern Art and Northern Ginseng". Atractylodes macrocephala polysaccharide (PAM) is a water-soluble polysaccharide extracted from Atractylodes macrocephala plant raw materials, and its mai...
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