Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A fluorescent oxygen nanosensor with mitochondrial targeting

A nanosensor, mitochondrial technology, applied in nanotechnology, nanotechnology, fluorescence/phosphorescence, etc., to achieve long luminescence lifetime, large stokes displacement, and improved biocompatibility

Inactive Publication Date: 2016-03-16
BEIJING JIAOTONG UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific fluorescence detection of dissolved oxygen in mitochondria is still a challenging task.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A fluorescent oxygen nanosensor with mitochondrial targeting
  • A fluorescent oxygen nanosensor with mitochondrial targeting
  • A fluorescent oxygen nanosensor with mitochondrial targeting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Dissolve PtTFPP, PS and DTS in tetrahydrofuran at a mass ratio of 2:48:50, and make their total concentration in the solution 0.2%. Then, using a micro-adjusting syringe, take 500 μL of the above solution, and quickly inject it into deionized water with a pH value of 9 (adjust the pH value with ammonia water) under the condition of ultrasonic vibration, and the resulting suspension is subjected to ultrasonic vibration for 2 hours, and then Dialyzed in secondary water for 24 hours, the nanoparticles used for measuring the oxygen content outside the cells were obtained.

[0024] (2) A mixed solution of the same concentration of PtTFPP, PS and DTS was quickly injected into deionized water with a pH value of 9 and a PLL concentration of 0.02mg / ml under the condition of ultrasonic vibration, and the resulting suspension was ultrasonically oscillated for 2 hours. , followed by dialysis in secondary water for 24 hours (to remove the organic solvent and excess PLL), the PLL...

Embodiment 2

[0036] Repeat Example 1, the difference is only platinum (II) MESO-tetrakis (pentafluorobenzene) porphine molecule, polystyrene and dodecyltrimethoxysilane mass ratio is according to 1:49:50, more The concentration of polylysine in deionized water is 0.01 mg / ml.

Embodiment 3

[0038] Repeat Example 1, the only difference is that the mass ratio of platinum (II) MESO-tetrakis(pentafluorobenzene) porphine molecule, polystyrene and dodecyltrimethoxysilane is 3:47:50.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a fluorescent oxygen nano sensor with cellular mitochondrion targeting. The nano sensor has the fluorescent service life sensitive to dissolved oxygen quantity, and can specifically target a mitochondrion in a cell. After the cell phagocytizes the oxygen nano sensor, variations of the dissolved oxygen quantity in a cytoplasm and the mitochondrion can be detected by a variation of the fluorescent service life of the nano sensor. The fluorescent oxygen nano sensor with the cellular mitochondrion targeting has the following characteristics that (1) a PLL (Phase Locked Loop) housing layer allows the sensor to have good organism compatibility; encapsulation allows a probe to have external interference resistance; (2) the mitochondrion targeting is good; (3) the oxygen sensitivity of the fluorescent service life is high; (4) the dissolved oxygen quantity in different positions in the cell can be detected.

Description

technical field [0001] The invention belongs to the technical field of fluorescent detection of biological dissolved oxygen, and in particular relates to a fluorescent oxygen nanometer sensor with cell mitochondria targeting and a preparation method thereof. Background technique [0002] Mitochondria are important organelles in cells and play an important role in maintaining the homeostasis of physiological functions. Mitochondrial dysfunction is directly related to a series of pathological diseases, such as obesity, diabetes, tumors, and cardiovascular diseases. Therefore, the detection of mitochondrial function in cells is of great significance. As we all know, mitochondria are the main place for intracellular aerobic respiration to generate energy, and most of the oxygen in cells is utilized by mitochondria. If the dissolved oxygen level in mitochondria can be monitored in real time, it can indirectly reflect the activity of mitochondria and its metabolic function. [0...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64B82B3/00
Inventor 彭洪尚王小卉
Owner BEIJING JIAOTONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com