Preparation method for Picris japonica extract and application thereof

A technique for the preparation of Chinese herbal medicine extracts, the preparation of medicines for preventing and treating diabetes, and the field of preparation of the extracts of the lotus root can achieve the effects of low production cost and simple operation of the preparation process.

CN103585226AInactive Publication Date: 2014-02-19SHANDONG RES INST OF TUMOUR PREVENTION TREATMENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2014-02-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a preparation method for a Picris japonica extract and application thereof. The preparation method includes: extracting Picris japonica, conducting high speed counter current chromatography, polyamide and other chromatography purification to obtain a Picris japonica extract B and a Picris japonica extract C, and mixing the Picris japonica extract B with the Picris japonica extract C in certain proportion so as to obtain the Picris japonica extract, which can be combined with a pharmaceutically acceptable carrier or other suitable excipients so as to be prepared into oral medication dosage forms according to conventional methods. The Picris japonica extract can be used for preparing drugs for prevention and treatment of diabetes.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, and relates to a preparation method and application of an extract of Chinese medicinal materials, in particular to a preparation method of a lotus root extract and its application in the preparation of drugs for preventing and treating diabetes. Background technique

[0002] With the aging of the world's population, diabetes has become a common and frequently-occurring disease, and its incidence is on the rise in industrialized countries. According to statistics, there are about 150 million diabetic patients in the world, 80% of which are non-insulin-dependent diabetes (type 2), of which there are about 30 million in my country. The number of deaths caused by diabetes complications ranks third after cardiovascular and cerebrovascular diseases and cancer in developed countries, which has attracted great attention from all over the world, and researches on the pathogenesis and drug prevention and treatme...

Examples

Embodiment 1

[0030] Example 1: Preparation of the lotus extract and monomer compound

[0031] (1) 30kg of lotus root, use 65% ethanol as the solvent, extract by warm soaking at 25°C, the number of extractions is 3 times, each extraction time is 24 hours, and the amount of solvent used each time is 23 times the weight of the medicinal material; filter , combining the extracts, recovering ethanol, concentrating, and drying to obtain the lotus root extract A;

[0032] (2) Add 15 times the amount of water to dissolve the lotus root extract A obtained in step (1), and filter to obtain medicinal solution A and precipitation A; use high-speed countercurrent chromatography (HSCCC) to dissolve precipitation A with petroleum ether -Ethyl acetate-methanol-water (2:2:1:1) is the solvent system, separation and purification are carried out, the solvent is recovered, concentrated and dried, and the lotus root extract B is obtained;

[0033] (3) Pass the drug solution A obtained in step (2) through a pol...

Embodiment 2

[0037] Embodiment 2: Preparation of lotus root extract and monomer compound

[0038] (1) 10 kg of lotus root, use ethanol with a concentration of 65% as a solvent, and extract by warm soaking at 10°C, the number of extractions is 5 times, each extraction time is 48 hours, and the amount of each solvent is 25 times the weight of the medicinal material; filter , combining the extracts, recovering ethanol, concentrating, and drying to obtain the lotus root extract A;

[0039] (2) Dissolve the lotus extract A obtained in step (1) with 20 times the amount of water, and filter to obtain liquid A and precipitation A; use high-speed countercurrent chromatography (HSCCC) to dissolve precipitation A with petroleum ether -Ethyl acetate-methanol-water (2:2:1:1) is the solvent system, separation and purification are carried out, the solvent is recovered, concentrated and dried, and the lotus root extract B is obtained;

[0040] (3) Pass the drug solution A obtained in step (2) through a p...

Embodiment 3

[0044] Embodiment 3: Preparation of lotus root extract and monomer compound

[0045] (1) 10kg of hair lotus, use 65% ethanol as solvent, and extract by warm soaking at 35°C, the number of extractions is 1 time, each extraction time is 12 hours, and the amount of each solvent is 21 times the weight of the medicinal material; filter , combining the extracts, recovering ethanol, concentrating, and drying to obtain the lotus root extract A;

[0046] (2) Dissolve the lotus root extract A obtained in step (1) with 5 times the amount of water, and filter to obtain the medicinal solution A and precipitation A; the precipitation A is mixed with petroleum ether by high-speed countercurrent chromatography (HSCCC). -Ethyl acetate-methanol-water (2:2:1:1) is the solvent system, separation and purification are carried out, the solvent is recovered, concentrated and dried, and the lotus root extract B is obtained;

[0047] (3) Pass the drug solution A obtained in step (2) through a polyamid...