The invention relates to the field of animal nutrition, and particularly relates to a vitamin premix feed for laying hens and a preparation method thereof. The vitamin premix feed for the laying henscomprises the following ingredients: vitamin A, VD3, dl-alpha-tocopherol acetate, menadione sodium bisulfite (MSB), thiamine nitrate, riboflavin, pyridoxine hydrochloride, cyanocobalamin, nicotinamide, D-calcium pantothenate, folic acid, and D-biotin; and the vitamin premix feed for the laying hens is added into a compound feed for laying hens in laying periods in a mass percentage of 0.03%. The vitamin premix feed for the laying hens facilitates absorption and digestion of laying hens to various feed nutrients, and is capable of enhancing immune functions of laying hens, preventing various diseases in growth processes, improving survival rates, increasing appetite, digestion and absorption of the laying hens, ensuring stable egg production of the laying hens at all seasons, improving eggshell quality and prolonging egg production period.