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A drug combination for the treatment of type 2 diabetes and its complications

A diabetes and anti-diabetic technology, applied in the field of medicine, can solve the problems of berberine's clinical efficacy and insufficient treatment range

Active Publication Date: 2019-05-24
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to solve the deficiencies of the existing single compound berberine in clinical efficacy and therapeutic range, and provide a kind of berberine and licorice extract (or glycyrrhizic acid) in which the therapeutic effect of diabetes and its complications is better than that of a single compound pharmaceutical composition

Method used

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  • A drug combination for the treatment of type 2 diabetes and its complications
  • A drug combination for the treatment of type 2 diabetes and its complications
  • A drug combination for the treatment of type 2 diabetes and its complications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, the preparation of licorice extract.

[0023] 1. Experimental materials and methods.

[0024] Licorice raw materials were purchased from Beijing Tianheng Pharmacy; deionized water: Purified by Milli-Q system (Millipore, MA, USA).

[0025] 1. Preparation method:

[0026] Add water 8 times the weight of the raw material of licorice Chinese medicine into the container, soak for 0.5-3 hours, decoct for 1-2 hours, and filter to obtain the filtrate (1); add water 8 times the weight of the raw material of licorice Chinese medicine, decoct for 1-2 hours Filtrate for 2 hours to obtain filtrate (2); add water 8 times the weight of licorice traditional Chinese medicine raw material into the container, decoct for 0.5-1.5 hours, and filtrate to obtain filtrate (3). All filtrates were combined and concentrated under reduced pressure to obtain licorice extract.

[0027] 2. Fingerprint detection conditions:

[0028] Detection system: Agilent1260 liquid phase system. ...

Embodiment 2

[0031] Example 2. Effects of the pharmaceutical composition on blood glucose and insulin in db / db spontaneously diabetic mice.

[0032] 1. Experimental materials and methods.

[0033] 1. Experimental drugs and reagents

[0034] The reference substances of glycyrrhizic acid and berberine were purchased from Chengdu Mansite Biotechnology Co., Ltd.; the positive drug metformin was purchased from Beijing Kulaibo Technology Co., Ltd. The purity of the compound reached 98% by HPLC / UV analysis. Licorice raw materials were purchased from Beijing Tianheng Pharmacy. Mouse Insulin ELISA kit (Shanghai Yikesai Biological Products Co., Ltd.).

[0035] 2. Experimental animals

[0036] In this study, db / db male mice (permit number: SCXK (Su) 2011-0003), 8 weeks old, purchased from Changzhou Cavens Experimental Animal Co., Ltd., were used in the M3 mouse cage (length × width × Height = 318×202×135mm), drinking water supply is sufficient, and feed and tap water are freely ingested. The roo...

Embodiment 3

[0053] Example 3. The effect of the pharmaceutical composition on blood lipids in db / db spontaneously diabetic mice.

[0054] 1. Experimental materials and methods.

[0055] The reference substances of glycyrrhizic acid and berberine were purchased from Chengdu Mansite Biotechnology Co., Ltd.; the positive drug metformin was purchased from Beijing Kulaibo Technology Co., Ltd. The purity of the compound reached 98% by HPLC / UV analysis. Licorice raw materials were purchased from Beijing Tianheng Pharmacy.

[0056] The use of db / db male mice in this study is consistent with Example 2. Detection of serum biochemical indicators: determination of total cholesterol (CHOL), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglyceride (TG) in mouse serum.

[0057] Second, the experimental results.

[0058] By comparing the total cholesterol (CHOL) and high-density lipoproteins of the berberine group, the combination group of berberine and glycyrrhizic acid 1:1.1, t...

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Abstract

The invention, in accordance with the theoretical guidance of traditional Chinese medicine prescription, provides a pharmaceutical composition of berberine and a liquorice root extract as well as berberine and glycyrrhizic acid. Compared with single administration, the pharmaceutical composition disclosed by the invention has obvious advantages in the aspect of improving blood glucose, glycated serum protein and insulin levels of a mouse with diabetics, and the pharmaceutical composition has a good synergistic effect; in the aspect of improving lipid metabolism, reducing serum glutamic pyruvic transaminase (ALT) and glutamic oxalacetic transaminase (AST) levels and the like, the pharmaceutical composition also has a good effect, and the pharmaceutical composition has a synergistic effect; and in addition, the pharmaceutical composition is capable of significantly reducing a serum total creatinine (Cre) level and is capable of effectively improving renal functions. Therefore, the pharmaceutical composition can be used for preventing and / or treating diabetics and complications thereof; and the pharmaceutical composition has positive practical significance to the development of medicine.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the application of a pharmaceutical composition of berberine and licorice extract (or glycyrrhizic acid) in preventing and treating diabetes and its complications. Background technique [0002] Diabetes Mellitus (DM) is a group of clinical syndromes caused by the interaction of genetic and environmental factors, divided into type I (insulin-dependent diabetes mellitus, IDDM) and type II (insulin-independent diabetes mellitus, NIDDM), clinical The main sign is high blood sugar. Among them, type II diabetes accounts for more than 90% of diabetic patients, and is mainly related to two physiological defects, namely insulin secretion defect and insulin resistance. Insulin resistance refers to the decreased sensitivity and responsiveness of insulin peripheral tissues and their target organs or target tissues (mainly liver, adipose tissue, skeletal muscle) to insulin, resul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/704A61K36/484A61P3/10A61P1/16A61P3/06A61P13/12A61K31/4375
CPCA61K31/4375A61K31/704A61K36/484A61K2300/00
Inventor 叶敏王琦乔雪匡易
Owner PEKING UNIV
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