Human urothelial carcinoma specific antibody and its application
A technology for human urine and skin cancer, applied in the field of tumor immunology, can solve problems such as unsatisfactory sensitivity or specificity, nuclear matrix protein, telomerase and microsatellite instability
Active Publication Date: 2015-10-21
中科健兰(北京)医学研究院
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Problems solved by technology
Molecular markers used clinically in recent years, such as nuclear matrix protein, telomerase, and microsatellite instability, have unsatisfactory sensitivity or specificity
Method used
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Embodiment Construction
[0009] The present invention provides a hybridoma cell of a novel monoclonal antibody against human urothelial carcinoma TNFRSF25, and the monoclonal antibody DTUC secreted by it, and DTUC1 recognizes the epitope of TNFRSF25. DTUC1 antibody showed strong positive reaction with human urothelial carcinoma tissue and human urothelial cancer cell lines 5637, UMUC3, SW780, J82, T24 and RT4, but had no crossover with normal human urinary tract tissue and other non-urothelial cancer cells reaction. The present invention also provides an in vitro diagnostic kit comprising the monoclonal antibody DTUC1 and a method for detecting the content of urothelial carcinoma tumor markers in urine by using the monoclonal antibody DTUC1.
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The invention provides a new human urothelial carcinoma tumor marker-human tumor necrosis factor receptor superfamily TNFRSF25 and a hybridoma cell generating a monoclonal antibody resisting the tumor marker and a monoclonal antibody DTUC1 secreted thereby, wherein the monoclonal antibody DTUC1 is secreted by the hybridoma with collection number CGMCC No.8118. The monoclonal antibody DTUC1 is in positive reaction with the human urothelial carcinoma tissue and urothelial carcinoma cell lines such as 5637, UMUC3, SW780, J82, T24 and RT4, and is not in cross reaction with the human normal urothelium tissue and other non-urothelial carcinoma cells; according to mass spectrometric detection, the identified antigen is TNFRSF25. The invention also provides an in-vitro diagnosis kit comprising the monoclonal antibody DTUC1 and a method for detecting the content of tumor markers in urine by use of the monoclonal antibody DTUC1.
Description
technical field [0001] The invention belongs to the field of tumor immunology. Specifically, the present invention relates to a monoclonal antibody DTUC1 against human urothelial carcinoma TNFRSF25. The antibody can specifically recognize human urothelial carcinoma TNFRSF25 at biochemical, cellular and tissue levels. The invention also relates to a double-antibody sandwich ELISA method for detecting human urothelial carcinoma. The stationary phase in this detection method is B-8 antibody (purchased from Santa Cruz Biotechnology, Inc.), and the detection antibody is DTUC1 in the present invention, which is used to detect the content of tumor marker TNFRSF25 in urine. Background technique [0002] Urothelial carcinoma refers to tumors occurring in epithelial cells covering the urinary tract, including bladder urothelial carcinoma, ureter urothelial carcinoma, and renal pelvis urothelial carcinoma, which account for the vast majority of urinary system tumors, among which blad...
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IPC IPC(8): C07K16/30G01N33/68G01N33/574C12N5/20C12R1/91
CPCC07K16/2878C12N5/163G01N33/57407G01N33/577G01N2800/34
Inventor 李翀栗刚
Owner 中科健兰(北京)医学研究院