Tissue-culture rapid propagation method of cymbidium
A technology of tissue culture rapid propagation and medium, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems affecting the profit margin of orchid tissue culture seedlings, high medium cost, long tissue culture cycle, etc., to achieve The effect of shortening the seedling time, saving dosage and simplifying the production procedure
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[0014] A method for tissue culture and rapid propagation of Cymbidium cymbidium, comprising the steps of:
[0015] (1) Induction of protocorm of Cymbidium pendantii: the culture medium is 40% pine bark + 60% medium; the medium is 1 / 2MS basic medium, sugar 30g / L, agar 5-8g / L, pH5. 6. The culture medium was sterilized at 121°C and 1.1kg / cm2 for 18 minutes. Take the uncracked Cymbidium pendulum capsule, first treat it with 70% to 75% ethanol for 1 to 1.5 minutes, then dip it in 95% ethanol and burn it on the flame of an alcohol lamp for 2 to 3 times; cut open the sterilized capsule, put Sprinkle the seeds into sterile water, shake them well, and then inoculate them on the culture substrate; the culture temperature is 25±2°C, the light intensity is 1500-2000Lx, and the light-dark cycle ratio is 12h:12h. After 40 days of cultivation, green protocorms can be induced to form.
[0016] (2) Proliferation culture of Cymbidium pendulatum protocorm: culture medium is 40% pine bark + 60%...
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