Tissue-culture rapid propagation method of cymbidium

A technology of tissue culture rapid propagation and medium, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems affecting the profit margin of orchid tissue culture seedlings, high medium cost, long tissue culture cycle, etc., to achieve The effect of shortening the seedling time, saving dosage and simplifying the production procedure

Active Publication Date: 2014-03-05
湖州千鑫物资贸易有限责任公司
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Problems solved by technology

Since the development of orchid tissue culture in the 1960s, it has been greatly developed and a relatively complete technical system has been established.

Method used

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Embodiment 1

[0014] A method for tissue culture and rapid propagation of Cymbidium cymbidium, comprising the steps of:

[0015] (1) Induction of protocorm of Cymbidium pendantii: the culture medium is 40% pine bark + 60% medium; the medium is 1 / 2MS basic medium, sugar 30g / L, agar 5-8g / L, pH5. 6. The culture medium was sterilized at 121°C and 1.1kg / cm2 for 18 minutes. Take the uncracked Cymbidium pendulum capsule, first treat it with 70% to 75% ethanol for 1 to 1.5 minutes, then dip it in 95% ethanol and burn it on the flame of an alcohol lamp for 2 to 3 times; cut open the sterilized capsule, put Sprinkle the seeds into sterile water, shake them well, and then inoculate them on the culture substrate; the culture temperature is 25±2°C, the light intensity is 1500-2000Lx, and the light-dark cycle ratio is 12h:12h. After 40 days of cultivation, green protocorms can be induced to form.

[0016] (2) Proliferation culture of Cymbidium pendulatum protocorm: culture medium is 40% pine bark + 60%...

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Abstract

The invention belongs to the technical field of biology and particularly relates to a tissue-culture rapid propagation method of cymbidium. The method comprises the following steps: (1) induction and formation of protocorm of the cymbidium, wherein a culture base material is composed of 40% of pine barks and 60% of a culture medium and the culture medium is a 1/2MS basic culture medium; (2) multiplication culture of the protocorm of the cymbidium, wherein a culture base material is composed of 40% of pine barks and 60% of a culture medium and the culture medium is composed of 1/2MS, 1.0-2.0mg/L 6-BA, 0.3-0.8mg/L NNA, 0.3-0.8mg/L KT and 50g/L CW; (3) differentiation and strong seedling rooting of the protocorm of the cymbidium, wherein a culture base material is composed of 40% of pine barks and 60% of a culture medium and the culture medium is composed of 1/2MS, 0.5-1.2mg/L 6-BA, 0.1-0.3mg/L NNA and 100g/L PJ; and (4) domestication and transplanting of a cymbidium test tube seedling. According to the tissue-culture rapid propagation method of cymbidium, a cheap and efficient tissue-culture rapid propagation system of cymbidium is established.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for tissue culture and rapid propagation of Cymbidium cymbidium. Background technique [0002] The economic value of orchids is very high, and most of them are famous ornamental flowers and precious medicinal plants; after the Cymbidium came out in China, it has become even more kingly, and has been elected as the king of flowers in Guangzhou for many years. At present, except for breeding research at home and abroad, most commercial orchid propagation is carried out by tissue culture. Since the development of orchid tissue culture in the 1960s, it has been greatly developed and a relatively complete technical system has been established. However, the higher cost of medium and the longer tissue culture cycle have greatly affected the growth of orchid tissue culture seedlings. Profit margins. [0003] Bark is an organic waste produced during wood processing, wit...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈建中孙庆俊张海洋李百健
Owner 湖州千鑫物资贸易有限责任公司
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