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Method for transforming gingko pollen flavonoid glycoside employing microorganisms

A technology of microbial transformation and flavonoid glycosides, applied in the field of microorganisms, can solve problems such as no reported strain functions, and achieve the effects of improving bioavailability, strong fecundity, and rapid growth

Active Publication Date: 2014-03-05
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the function of this strain has not been reported

Method used

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  • Method for transforming gingko pollen flavonoid glycoside employing microorganisms

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Effect test

Embodiment 1

[0021] Example 1: Activation of Lactobacillus BL1 involved in the present invention and preparation of liquid strains

[0022] MRS solid medium is: peptone 5 g, beef extract 5 g, yeast extract 2.5 g, glucose 2.5 g, triammonium citrate 1.0 g, sodium acetate 1.56 g, potassium acetate 1.125 g , disodium hydrogen phosphate 0.815 g, manganese sulfate tetrahydrate 0.125 g, magnesium sulfate heptahydrate 0.29 g, Tween 80 is 0.5 ml, agar 10 g, distilled water 500 ml, pH6.5, 121 Sterilize at ℃ for 20 min.

[0023] MRS slant medium: same as MRS solid medium.

[0024] The liquid MRS medium is: remove the agar for the MRS solid medium components.

[0025]Streak culture on MRS solid medium, isolate a single colony and transfer to MRS slant medium, culture at 37°C for 24-48 hours to activate the strain; inoculate the slant into liquid MRS medium, cultivate at 37°C for 18-24 hours to obtain liquid Strains, the number of beneficial viable bacteria can reach 10 9 cfu / mL.

Embodiment 2

[0026] Embodiment 2: flavonoid glycoside biotransformation process involved in the present invention

[0027] Pass ginkgo pollen through a 100-mesh sieve, prepare the ratio of 20 parts by weight of ginkgo pollen and 100 parts by weight of water, and stir evenly to obtain a fermentation substrate; sterilize the fermentation substrate at 115°C for 15 minutes, cool to room temperature, and inoculate Lactobacillus at a volume ratio of 4%. BL1 liquid strains were cultured statically at 37°C for 4 days to obtain a high-efficiency transformation solution of ginkgo pollen flavonoid glycosides.

Embodiment 3

[0028] Embodiment 3: flavonoid glycoside biotransformation process involved in the present invention

[0029] Ginkgo pollen is passed through a 100-mesh sieve, prepared according to the ratio of 30 parts by weight of ginkgo pollen and 100 parts by weight of water, and stirred evenly to obtain a fermentation substrate; the fermentation substrate is sterilized at 115°C for 20 minutes, cooled to room temperature, and inoculated with Lactobacillus at a volume ratio of 2%. BL1 liquid strains were cultured statically at 34°C for 10 days to obtain a high-efficiency transformation solution of ginkgo pollen flavonoid glycosides.

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Abstract

The invention discloses a method for transforming gingko pollen flavonoid glycoside employing microorganisms. By adopting the method, the gingko pollen flavonoid glycoside is transformed by using lactobacillus BL1. The bacterial strain is facultative anaerobic, simple in cultivation condition, quick to grow, strong in fertility, safe and efficient, can generate a plurality of complex enzymes, and can carry out growth and propagation by taking single gingko pollen as matrix. The gingko pollen does not need wall-breaking treatment in advance; the flavonoid glycoside in the gingko pollen does not need complicated extraction and purification, and is efficiently transformed into corresponding aglycone; the capability of directly taking the gingko pollen as the raw material to transform the flavonoid glycoside into corresponding aglycone is high. The conversion ratio of flavonoid glycoside kaempferide-3,4'-diosgenin-diglucoside, 3-O-[6-O(alpha-L-rhamnose base)-beta-D-glucosyl] kaempferide and kaempferol-3-glucoside is up to over 99 percent. The kaempferol which is over 100 times more than the former can be obtained from the gingko pollen by transformation. Thus, the bioavailability is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for transforming ginkgo pollen flavonoid glycosides by microorganisms. Background technique [0002] Ginkgo tree, also known as "Gongsun tree", is the oldest relict plant among the existing seed plants. Ancient ginkgo trees are mainly distributed in China as wild resources, and then introduced to all parts of the world. At present, the main production areas of ginkgo in China are mainly in Shandong, Jiangsu, Guangxi, Guangdong and other places. The whole body of ginkgo is a treasure, and the utilization of ginkgo resources has a long history in my country. Ginkgo leaves and ginkgo fruit can be used as medicine, which are all recorded in "Chinese Pharmacopoeia". Ginkgo biloba contains natural active flavonoids and lactones and other ingredients that are beneficial to human health. It can dissolve cholesterol and expand blood vessels. It can improve br...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12R1/225
Inventor 陈相艳裘纪莹陈蕾蕾王未名刘孝永周庆新
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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