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Preparation method of effective polypeptide components in Polistidae insects, and medicinal uses of effective polypeptide components

An effective part, wasp family technology, applied in the field of medicine, can solve the problems of low industrial production supporting level, failure to form large-scale production capacity, etc., and achieve the effects of reducing cerebral edema, prolonging breathing time, and anti-platelet aggregation.

Inactive Publication Date: 2014-03-26
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with developed countries, there is still a relatively obvious gap in the research and development of my country's TTS system, especially the low level of supporting industrial production, which has not yet formed a large-scale production capacity

Method used

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  • Preparation method of effective polypeptide components in Polistidae insects, and medicinal uses of effective polypeptide components
  • Preparation method of effective polypeptide components in Polistidae insects, and medicinal uses of effective polypeptide components
  • Preparation method of effective polypeptide components in Polistidae insects, and medicinal uses of effective polypeptide components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 : Preparation of active parts of wasp venom polypeptide and film-coating agent

[0035] 1.1 Collection of crude wasp venom:

[0036] The spotted-winged wasp was collected from Pu'er City, Yunnan Province. The bee body specimen was identified as Polistes maculipennis Saussure (Polistes maculipennis Saussure) according to Professor Yang Zizhong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 10V , output automatic intermittent pulsating voltage; method of collecting wasp venom: place the venom collector in front of the hive, and collect venom from the worker bees of the spotted-winged wasp colony according to the routine poison co...

Embodiment 2

[0040] Example 2 : about wasp bee venom polypeptide effective fraction and preparation of hydrogel

[0041] 2.1 About the collection of wasp venom:

[0042] The wasps were collected from Guangnan County, Yunnan Province. The bee specimens were identified as Polistes jokahamae Rodoszkowski by Professor Guo Yunjiao of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 10V , output automatic intermittent pulsating voltage; method of collecting wasp venom: put the unpainted poison collector in front of the hive, and collect venom from the number of nests and worker bees according to the routine poison collection procedures. Turn on the power switch of t...

Embodiment 3

[0047] Example 3 : Preparation of the active part of the wasp bee venom polypeptide and its poultice

[0048] 3.1 Collection of crude venom of land hornets:

[0049] The land hornet was collected from Lijiang, Yunnan Province. The bee body specimen was identified as Polistes rothneyi grahami van der Vecht (the land hornet) according to Professor Yang Zizhong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. The basic device for poison collection uses a sub-cap type bee venom collector modified by the engineering research center, with a wooden frame, 80 cm long and 50 cm wide; 4 pieces of poisonous glass with a length of 20 cm / width of 33 cm are embedded; the input voltage is 9V, Output automatic intermittent pulsating voltage; The wooden frame is painted red with red paint in advance; The wooden frame is pre-smeared 20 milliliters of the honeycomb and honeycomb body extract volatile...

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Abstract

The invention relates to a preparation method of effective polypeptide components in Polistidae insects, and medicinal uses of the effective polypeptide components. The effective components are obtained through a step of dialyzing crude hornet venom of Polistidae hornet through a 25KDa dialysis membrane, and a step of freeze-drying or reduced pressure concentration drying of the above obtained dialysis liquid. A series of pharmacodynamic experiments verify that the effective biotoxin polypeptide components and transdermal absorption preparations thereof can substantially control the cerebral thrombus, can recover the mobility of cerebral palsy animals, can mitigate the ischemic brain injury, have stronger pharmacodynamic performances than first-line medicines comprising plavix, Xingnaojing and the like, and can be expected to be used for preparing biochemical medicines for preventing and treating cardiovascular and cerebrovascular diseases in order to prevent and treat ischemic cardiocardiovascular diseases, such as myocardial infarction, cardiovascular thrombus, cerebral thrombus, cerebral infarction, acute / chronic cerebral apoplexy, and sequelae caused by cerebral infarction injury, like lateral hemiplegia, hemidysesthesia, hemiopia, facial and lingual paralysis, aphasia and general paralysis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular, the invention relates to a preparation method of an effective part of a polypeptide in insects of the family Polistidae and its medical application. The effective part is obtained by dialysis of the crude bee venom of Vespa vespae with a dialysis membrane with a molecular weight cut-off of 25KDa, and the eluate is freeze-dried or concentrated and dried under reduced pressure. Through a series of pharmacological and pharmacodynamic experiments in animals, the inventors found that the effective part of the polypeptide has the effect of significantly preventing and treating cerebral thrombosis, restoring the activity ability of cerebral palsy animals, and reducing ischemic brain damage, and can be expected to be used in the preparation of anti-cardiac and cerebrovascular diseases. Vascular diseases include drugs, health care products, medical devices and daily chemicals for ischemic ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P9/10A61P7/02A61P25/00
Inventor 彭芳赵海荣吕小满郑园园巫秀美赵昱
Owner DALI UNIV
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