Inducing medium and method for improving survival rate of butterfly orchid pedicel
A technology of induction medium and culture medium, which is applied in cell culture medium, horticultural methods, botanical equipment and methods, etc., can solve the problems of large damage to explants, low survival rate of flower stems, and incomplete disinfection, so as to improve the survival rate. rate, inhibition of browning, and shortening of germination time
Inactive Publication Date: 2014-12-10
TODAYS LANDSCAPING BIOLOGICAL TECH
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Problems solved by technology
At present, in the process of using pedicel axillary buds as explants to induce the cultivation of Phalaenopsis, there are often problems such as incomplete disinfection during the initial induction culture, great damage to the explants during sterilization, and low survival rate of the pedicel.
Carbenicillin is an antibiotic, which is mostly used in medicine at present, mainly for inflammation caused by Pseudomonas aeruginosa, some Proteus, Escherichia coli, etc. It is rarely used in tissue culture applications
Method used
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Embodiment 1～7
[0028] 1) Preparation of the induction medium used in Examples 1-7
[0029] The induction medium of Examples 1 to 7 is prepared by adding agar, white granulated sugar, gac, coconut milk, carbenicillin, 6-benzylaminoadenine, naphthaleneacetic acid to 1 / 2 MS medium, agar, white granulated sugar, See Table 1 for the concentrations of activated carbon, coconut milk, carbenicillin, 6-benzylaminoadenine, and naphthaleneacetic acid in the induction medium of Examples 1-7. Induction medium numbers 1 to 7 in Table 1 correspond to the induction medium in Examples 1 to 7 respectively. After the induction media of Examples 1-7 were prepared, they were packed in No. 1-7 bottles respectively. The used 1 / 2MS medium, its formulation table is as shown in Table 2 below, the dosage described in Table 2 refers to the consumption when preparing 1 liter of 1 / 2MS medium, the consumption of macroelements in 1 / 2MS medium It is half of the macroelement dosage of the standard MS medium.
[0030] Tabl...
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The invention provides an inducing medium and a method for improving a survival rate of a butterfly orchid pedicel. The inducing medium is prepared by adding agar, white granulated sugar, 6-benzylaminopurine, naphthylacetic acid, carbenicillin, coconut milk and activated carbon in a 1 / 2 MS (Murashige-Skoog) culture medium. The inducing medium comprises the following ingredients at concentration: 7-9 g / L of agar, 15-30 g / L of white granulated sugar, 0-50 mg / L of carbenicillin, 1.5-3 mg / L of 6-benzylaminopurine, 0.2-0.5 mg / L of naphthylacetic acid, 0-100 g / L of coconut milk, and 1-3 g / L of activated carbon. The inducing medium can be used for inducing the butterfly orchid pedicel. The method for improving the survival rate of the butterfly orchid pedicel is low in cost, and low in the sterilizing contamination rate, and can effectively improves the survival rate of the sterilized explant.
Description
technical field [0001] The invention belongs to the technical field of plant tissue culture industrialized seedling cultivation, and in particular relates to a method for effectively improving the survival rate and induction effect of the Phalaenopsis pedicels when using the Phalaenopsis pedicels as explants for primary culture. Background technique [0002] Phalaenopsis B1.spp. is a tropical aerial orchid, which has large flowers, long flowering period, gorgeous flower color, rich color, beautiful and unique flower shape, dancing like a butterfly, hence the name. Known as the "Queen of Orchids" among tropical orchids, it is one of the most popular catayas in recent years. [0003] Phalaenopsis is a single-stem aerial orchid, and the plants rarely develop side buds, and the seeds are extremely difficult to germinate. Its conventional propagation is very slow, so tissue culture is often used for rapid propagation. Disinfection of explants is the first step in tissue rapid pr...
Claims
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IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/005A01H4/008C12N5/0025A01H4/002
Inventor 陈永得
Owner TODAYS LANDSCAPING BIOLOGICAL TECH