Fermentation process of neomycin

A fermentation process and neomycin technology are applied in the biological field to achieve the effects of improving titer, improving fermentation production level and saving cost

Inactive Publication Date: 2014-04-02
YICHANG SANXIA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a fermentation process of neomycin, which solves the viscosity and abnormal metabolism of the fermentation liquid in the fermentation process, improves the metabolic state of the fermentation liquid and the level of fermentation production.

Method used

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  • Fermentation process of neomycin
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  • Fermentation process of neomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Seal the sandy soil spores, dry them, store them at 4°C for 10 days, then place them at 28±2°C for 10 days to prepare the inclined plane spore suspension;

[0034] 2. The slant spore suspension was put into a seed tank with a seed medium set in advance and cultivated for 48 hours to obtain a culture solution. The mass content of soybean powder in the seed tank was 3.3% of the seed medium.

[0035] 3. Send the seed culture liquid that obtains through step 2 into the fermentor that pre-sets fermentation medium to inoculate, wherein the mass content of glucose is 1.0% of the fermentation medium quality, and the mass content of corn steep liquor is 0.18% of the fermentation medium quality %, fermented at 30-35°C to obtain a fermented liquid; after the seed culture liquid enters the fermenter, air is introduced, and the flow rate of the air is controlled to 500 3 / h, control the inlet for 9h; control the air inlet flow rate to 1600m 3 / h, control the access time to 29h, ...

Embodiment 2

[0040] 1. Seal the sandy soil spores, dry them, store them at 0°C for 5 days, then place them at 28±2°C, and cultivate them for 12 days to prepare the inclined plane spore suspension;

[0041] 2. Put the slant spore suspension into a seed tank with a seed medium set in advance and cultivate it for 36 hours to obtain a culture solution. The mass content of soybean powder in the seed tank is 1.3% of the seed medium.

[0042] 3. Put the seed culture solution obtained in step 2 into a fermenter with a pre-set fermentation medium for inoculation, and ferment at 30-35°C to obtain a fermentation solution. After the seed culture solution enters the fermenter, air is introduced to the Air inlet flow control is 680m 3 / h, and pass through for 16h; increase the flow rate of air, and control the flow rate of air flow to 16000m 3 / h, and feed in for 22h, and add sugar; further increase the air intake, and control the air intake flow rate to 3000m 3 / h, and pass through for 42h, and contr...

Embodiment 3

[0047] 1. Seal the sandy soil spores, dry them, store them at 2°C for 7 days, and then culture them at 28±2°C for 8 days to prepare the inclined plane spore suspension;

[0048] 2. Put the slant spore suspension into a seed tank with a seed medium set in advance and cultivate for 40 hours to obtain a culture solution. The mass content of soybean powder in the seed tank is 2.4% of the seed medium.

[0049] 3. Put the seed culture solution obtained in step 2 into a fermenter with a pre-set fermentation medium for inoculation, and ferment at 30-35°C to obtain a fermentation solution. After the seed culture solution enters the fermenter, air is introduced to the Air inlet flow control is 500m 3 / h, and pass through for 30h; increase the flow of air, and control the flow rate of air flow to 1500m 3 / h, and feed in for 30h, and add sugar; further increase the air intake, and control the air intake flow rate to 2800m 3 / h, and pass through for 50h, and control the viscosity of the ...

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Abstract

The invention provides a fermentation process of neomycin. Cant spore suspension is put in a seed tank with pre-accommodated with a seed culture medium for cultivating 36-48 hours to obtain a culture solution; the seed culture medium in the seed tank contains soybean meal; the obtained seed culture solution is fed in a fermentation tank pre-accommodated with a fermentation culture medium for inoculating and fermenting 110-130 hours; a sugar material is supplemented in the fermentation process; the fermentation of the seed culture solution is realized through the phase control of air dosage and the volume control of fermentation solution; the cultivation of the fermentation solution is terminated; the fermentation solution is put in an absorption tank; 732 resins are added in the fermentation solution; the concentration of the fermentation solution is diluted below 13,000 u / ml; the pH value is neutralized within 6.4-7.0; then dilute fermentation solution is prepared; the fermentation steps are finished. The process improves the fermentation level above 15%, prominently creates the economic benefit, reduces the fermentation cost, and can be applied to the industrial production in large quantities.

Description

technical field [0001] The invention relates to a neomycin fermentation process and belongs to the field of biotechnology. Background technique [0002] There are many types of nitrogen source materials used in domestic neomycin fermentation. Our company has improved its process. Since the new formula (improved process) of neomycin fermenter was put into the tank, the production has the following disadvantages Stable factors make it difficult to achieve real stable production. For example, the viscosity of the fermentation broth is not enough in the early stage, the logarithmic growth phase of the bacteria is longer, and the sugar consumption rate is slower. Analyzing the reason, neomycin fermentation with new formula production tank, not only to optimize the formula of the fermenter, but also to optimize a series of processes in the whole process of fermentation production, such as the quality control of seed liquid, the control of intermediate feeding, etc. The process i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/52
Inventor 沈联兵姚明高郑兴
Owner YICHANG SANXIA PHARMA
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