Method for determining acquisition quality of blood sample used for science research

A blood sample and scientific research technology, which is applied in the field of blood sample quality judgment, can solve the problems of long-term room temperature exposure, influence, repeated freezing and thawing, etc., and achieves the effect of less sample consumption, simple and easy method, and fast speed.

Active Publication Date: 2014-04-16
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Due to the ubiquity of small molecule metabolites in various biological samples, the applicant found in previous research that metabolites in blood are susceptible to long-term room temperature exposure, hemolysis, and repeated freezing and thawing. These problems are also reported in the current literature. Many factors closely related to the quality of blood samples can affect the results of proteomics and metabolomics research

Method used

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  • Method for determining acquisition quality of blood sample used for science research
  • Method for determining acquisition quality of blood sample used for science research
  • Method for determining acquisition quality of blood sample used for science research

Examples

Experimental program
Comparison scheme
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Embodiment

[0021] 1 sample collection:

[0022] Group A of plasma samples from healthy adults (10 persons) was provided by Tuebingen University School of Medicine, Germany. Each sample was sampled in 500 μL according to clinical standard procedures. After sampling, the samples were divided into 5 groups, and each group contained 10 samples. The sample grouping includes 5 groups of 0 hours, 2 hours, 4 hours, 8 hours and 24 hours at room temperature.

[0023] 2 sample analysis

[0024] The chromatographic analysis adopts Agilent 1200 series rapid resolution liquid chromatography (Rapid Resolution Liquid Chromatography, RRLC), and the reversed-phase chromatographic column adopts 10cm×2.1mm 1.8μm ZORBAX TM SB-AQ C 18 column, the column temperature was maintained at 35°C. Mobile phase A was water containing 0.1% formic acid and mobile phase B was acetonitrile. The elution gradient started at 2% B, held for 1.5 minutes, increased to 30% in 4 minutes, and then reached 100% in 25 minutes. ...

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Abstract

The invention discloses a method for determining blood sample quality in a biological sample bank by utilizing two kinds of endogenous micromolecule metabolite, and belongs to the field of analytical chemistry application research. The group of metabolism markers is applicable to determine the quality of serum (or blood plasma) samples used for various scientific researches. The detection method is based on the metabonomics technology of high performance liquid chromatography-mass spectrometry combined technology, and after serum metabonomics data is acquired, in dependent on the relative content of hypoxanthine and sphingosine-1-phosphate in different-group samples, whether the sample quality problem caused by long-term room-temperature exposure during blood sample acquisition process exists can be determined. The method provided by the invention is beneficial to improve the quality of the blood samples in scientific research application, helps to guarantee accomplishment achieved in subsequential scientific research work and avoid manpower and material resources wastes caused by sample problems. No same kind methods are reported yet at present.

Description

technical field [0001] The invention relates to the application fields of analytical chemistry and metabonomics, and is a method for judging the quality of blood samples by detecting the levels of a group of blood small molecule metabolic markers. technical background [0002] At present, research in the field of life sciences pays more attention to the clinical application of new technologies and methods, so that human health can benefit from the rapid development of science and technology. In this process, the collection of clinical samples (blood, urine, tissue, etc.) is regarded as a key link, and it is also one of the most precious resources for the development of science and technology in various countries. However, since hospitals (sampling) and laboratories (scientific research) usually operate independently, for scientific researchers, sample quality problems caused by human factors in the sampling process may have unpredictable effects on experimental results , or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 尹沛源雷纳·莱曼黄强周丽娜赵欣捷路鑫许国旺
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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