Application of SIRT1 expression interfering agent in preparation of medicine for inhibiting self renewal of liver cancer stem cell agent
A technology for inhibiting liver cancer and stem cells, applied in the field of chemistry, can solve problems such as unreported, and achieve the effect of prolonging survival time and delaying tumor growth
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[0030] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. For the experimental methods that do not indicate the specific conditions in the examples, usually follow the conventional conditions, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), Shan et al.2012, hepatology, 56 : Conditions described in 1014-1014 or as recommended by the manufacturer.
[0031] The liver cancer stem cell model used in the present invention was constructed by our laboratory. The model contains a lentiviral expression vector (Nanog / GFP reporter system) constructed by the Nanog promoter regulating the GFP reporter gene. For the construction method, see Shan et al.2012, hepatology, 56: 1014-1014; Lv-sh-Scramble plasmid and Lv-sh-SIRT1 plasmid, adenovirus vector AD-GFP were constructed by our laboratory, see Shan et al.2012, Hepatology, 56: 1014-1014; HEK293 cells ...
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