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Reagent for quantitative detection of serum alpha 1-antitrypsin

An antitrypsin and quantitative detection technology is applied in the field of serum α1-antitrypsin quantitative detection reagents, which can solve the problems of inappropriate diagnosis of emergency patients and clinical patients, cumbersome operations, and time-consuming, and achieves rapid and simple detection and sensitive detection. High rate and fast detection effect

Active Publication Date: 2014-04-30
PUREBIO LAB NINGBO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main methods for detecting α1-antitrypsin include: serum trypsin inhibition capacity test, one-way immunodiffusion method, rocket immunoelectrophoresis method and ELISA method, but all of them have the disadvantages of cumbersome operation and long time-consuming, so they are not suitable for emergency patients timely diagnosis of clinical patients

Method used

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  • Reagent for quantitative detection of serum alpha 1-antitrypsin
  • Reagent for quantitative detection of serum alpha 1-antitrypsin
  • Reagent for quantitative detection of serum alpha 1-antitrypsin

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Serum α1-antitrypsin quantitative detection reagent, including reagent R1, reagent R2 and calibrator. specific:

[0027] Reagent R1 includes: pH 5.0 buffer solution 20mM, surfactant 0.01g / L, reaction accelerator 0.1g / L, electrolyte 50mM, stabilizer 0.1g / L, bacteriostat 0.1g / L.

[0028] Reagent R2 includes: pH 6.0 buffer 20mM, anti-human α1-antitrypsin antibody 2.0g / L, electrolyte 50mM, stabilizer 0.1g / L, bacteriostat buffer 0.1g / L.

[0029] The calibrator includes: pH 6.0 buffer 20mM, human α1-antitrypsin 3.00g / L, electrolyte 50mM, stabilizer 0.1g / L, bacteriostat 0.1g / L.

[0030] Among them, the buffer is phosphate buffer, the surfactant is Tween20, the reaction accelerator is polyethylene glycol 1500, the electrolyte is sodium ion, the stabilizer is bovine serum albumin, and the anti-human α1-antitrypsin antibody is sheep anti- Human polyclonal antibody and antibacterial agent use sodium azide.

[0031] The preparation of the reagent R1, reagent R2 and calibrator is...

Embodiment 2

[0051] Serum α1-antitrypsin quantitative detection reagent, including reagent R1, reagent R2 and calibrator.

[0052] specific:

[0053] Reagent R1 includes: pH 6.0 buffer solution 30mM, surfactant 0.05g / L, reaction accelerator 2.0g / L, electrolyte 100mM, stabilizer 5.0g / L, bacteriostat 2.0g / L.

[0054] Reagent R2 includes: pH 7.0 buffer solution 40mM, anti-human α1-antitrypsin antibody 10.0g / L, electrolyte 100mM, stabilizer 0.1g / L, bacteriostat 0.1g / L.

[0055] The calibrator includes: pH7.0 buffer 40mM, human α1-antitrypsin 4.00g / L, electrolyte 100mM, stabilizer 5.0g / L, bacteriostat 1.0g / L.

[0056] Among them, the buffer is phosphate buffer, the surfactant is Tween40, the reaction accelerator is polyethylene glycol 2000, the electrolyte is potassium ions, the stabilizer is gelatin, the antibacterial agent is potassium sorbate, and the antihuman α1-antipancreatic Rabbit anti-human polyclonal antibody was used as protease antibody.

[0057] The preparation of the reagent R1...

Embodiment 3

[0070] Serum α1-antitrypsin quantitative detection reagent, including reagent R1, reagent R2 and calibrator.

[0071] specific:

[0072] Reagent R1 includes: pH7.5 buffer 150mM, surfactant 1.0g / L, reaction accelerator 6.0g / L, electrolyte 250mM, stabilizer 10.0g / L, bacteriostat 5.0g / L;

[0073] Reagent R2 includes: pH 8.0 buffer solution 150mM, anti-human α1-antitrypsin antibody 50.0g / L, electrolyte 250mM, stabilizer 10.0g / L, bacteriostat 5.0g / L;

[0074] Calibrators include: 150mM buffer solution at pH 7.5, 250mM electrolyte, 10.0g / L stabilizer, 4.50g human α1-antitrypsin, and 5.0g / L antibacterial agent.

[0075] Among them, the buffer is Tris buffer, the surfactant is Triton X-100, the reaction accelerator is polyethylene glycol 4000, the electrolyte is sodium ion and magnesium ion, the stabilizer is bovine serum albumin, and the antibacterial agent is Proclin300. The anti-human α1-antitrypsin antibody used rabbit anti-human polyclonal antibody.

[0076] The preparation of...

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Abstract

The invention discloses a reagent for quantitative detection of serum alpha 1-antitrypsin. The reagent comprises a reagent R1, a reagent R2 and a calibrator. The reagent R1 comprises a buffer, a surfactant, a reaction promoter, an electrolyte, a stabilizing agent and an antibacterial agent. The reagent R2 comprises a buffer 2, an anti-human alpha 1-antitrypsin antibody, an electrolyte, a stabilizing agent and an antibacterial agent. The calibrator comprises a buffer, a human alpha 1-antitrypsin, an electrolyte, a stabilizing agent and an antibacterial agent. The reagent has the advantages of fast detection, high detection sensitivity, linearity range of 4.00g / L, high accuracy and good stability.

Description

technical field [0001] The invention relates to the technical field of detection reagents, in particular to a quantitative detection reagent for serum α1-antitrypsin. Background technique [0002] α1-antitrypsin (AAT), also known as α1 trypsin inhibitor, is a member of the serine protease inhibitor family. It is a single-chain glycoprotein composed of a polypeptide chain connecting three oligosaccharide chains, with a molecular weight of about 52kDa, PI The value is 4.8. As the most important protease inhibitor in the human body, α1-antitrypsin is mainly synthesized by liver cells, and can inhibit more than 90% of the protease activity in normal plasma. It can mainly inhibit neutrophil elastase, in all serine Among protease inhibitors, α1-antitrypsin has stronger vascular permeability, so the concentration of α1-antitrypsin in lung tissue is higher than that of other serine protease inhibitors, and it is more specific to elastase, mainly Inhibit the activity of trypsin and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31G01N21/82
CPCG01N33/5306G01N33/536G01N33/6893G01N2333/8125
Inventor 卓伟奇许国和范翠翠胡露群
Owner PUREBIO LAB NINGBO
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