A kit for diagnosing hepatocellular carcinoma

A technology of hepatocellular carcinoma and a kit, which is applied in the field of biomedicine, can solve problems such as abnormal protein content and reduced expression level of PON1, and achieve rapid, accurate, high-throughput, and high-specificity detection

Active Publication Date: 2016-03-30
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It was recently discovered that the protein content of HDL particles and some lipids is abnormal, including the glycosylation of PON1, which can lead to a decrease in the expression level of PON1 in the serum of patients with liver disease

Method used

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  • A kit for diagnosing hepatocellular carcinoma
  • A kit for diagnosing hepatocellular carcinoma
  • A kit for diagnosing hepatocellular carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] 1. A test kit for diagnosing hepatocellular carcinoma, comprising the following components:

[0027] 1. Fuc-PON1 semi-quantitative detection ELISA plate, the Fuc-PON1 semi-quantitative detection ELISA plate is composed of AAL-ELISA plate and Protein-ELISA plate.

[0028] 2. HCC prediction model:

[0029] P=exp(-0.915+0.275*Fuc-PON1) / [1+exp(-0.915+0.275*Fuc-PON1)]

[0030] The calculation method of the Fuc-PON1 value is: the ratio of the actual OD value of PON1 combined with AAL in each serum sample to the actual OD value of PON1 protein in the same serum sample, the actual OD of PON1 combined with AAL in the serum sample The value is obtained by AAL-ELISA plate detection, and the actual OD value of PON1 protein in serum samples is obtained by Protein-ELISA plate detection. The cutoff value of this model for distinguishing liver cancer and liver cirrhosis is 2.2727.

[0031]3. PON1 capture antibody, the concentration of which is 1 μg / mL. (R&D Systems, Catalog#DYC5816,...

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Abstract

The invention provides a kit for diagnosing hepatocellular carcinoma, which is characterized in that it comprises a Fuc-PON1 semi-quantitative detection ELISA plate, and the Fuc-PON1 semi-quantitative detection ELISA plate consists of an AAL-ELISA plate and a Protein- ELISA plate composition. The kit of the invention has prominent advantages such as high throughput, high specificity, fast and accurate detection, and the like, and has important clinical value for the diagnosis of liver cancer.

Description

technical field [0001] The invention relates to the field of biomedicine and is connected with applied basic medical research and clinical research; covering oncology, diagnostics and glycobiology. Background technique [0002] HCC is a common malignant tumor with multiple genes, multiple factors and complex pathological mechanisms. In my country, hepatitis B virus carriers account for 7.18% of the population, which is 2 / 3 of the world's hepatitis B carriers. The number of HCC patients related to this is huge, accounting for about half of the world. The vast majority of patients have insidious onset, and they are already at an advanced stage when they seek medical treatment after the appearance of clinical symptoms, losing the opportunity for treatment. Therefore, early diagnosis is very important to improve the survival rate of patients. An ideal liver cancer marker needs to have high specificity and be able to distinguish liver cancer from liver cirrhosis, hepatitis, liv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/96
CPCG01N33/57438G01N2800/085
Inventor 张舒
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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