A kind of biocontrol bacteria multi-effect compound preparation for disease resistance and production increase and preparation method thereof

A technology of composite preparations and biocontrol bacteria, applied in botany equipment and methods, biocides, fungicides, etc., can solve the problems of high spore mortality, short shelf life, dust scattering, etc., and achieve strong antagonism and passage stability Strong, high fermentation bacteria

Active Publication Date: 2016-01-06
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our country's research on the control of plant diseases by Bacillus subtilis has reached the world's advanced level. Now the products successfully developed and produced on a small scale include Yabao, Baikang, Maifengning, Wenquning, etc., which are used in the field to prevent and control cucumber powdery mildew , strawberry gray mold, wheat and rice sheath blight, etc., its effect is better than or equivalent to commonly used chemical fungicides, the main active ingredient of Bacillus subtilis for various purposes is living organisms, so compared with chemical pesticides, Formulation processing is more difficult. Bacillus subtilis is relatively backward in the development of formulations for biocontrolling Bacillus subtilis. Now there are mainly wettable powders, suspension concentrates and oils. Although these formulations have their own advantages, they still have high spore mortality, short shelf life, There are many shortcomings that cannot be overcome by themselves, such as unsatisfactory bactericidal effect, scattered dust, low adhesion to crops, and increased agricultural costs.

Method used

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  • A kind of biocontrol bacteria multi-effect compound preparation for disease resistance and production increase and preparation method thereof
  • A kind of biocontrol bacteria multi-effect compound preparation for disease resistance and production increase and preparation method thereof
  • A kind of biocontrol bacteria multi-effect compound preparation for disease resistance and production increase and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The concrete preparation method of embodiment 1 is as follows:

[0047] A. Preparation of fermentation broth

[0048] Put Bacillus subtilis Bs-16 and Bacillus subtilis Bs-20 in the pre-prepared amount of the existing NYDA culture solution and leave to ferment for 40 hours to obtain the original liquid fermentation solution, the number of bacteria reaches 10 10 More than spores / ml,

[0049] B. Prepare materials

[0050] Prepare materials according to the formula listed in Table 1. All solid additives are passed through a 100-mesh sieve, sterilized by ultraviolet light, and liquid additives should be used as new as possible to avoid contamination;

[0051] C. emulsification

[0052] Take the main drug fermentation liquid, emulsifier and antifreeze (also co-emulsifier) ​​and place them in an emulsifier to stir and emulsify for 30 minutes;

[0053] D. Add additives

[0054] Add UV protective agent, antibacterial synergist, and adhesive to the emulsion in sequence every...

Embodiment 2

[0055] The concrete preparation method of embodiment 2 is as follows:

[0056] A. Preparation of fermentation broth

[0057] Put Bacillus subtilis Bs-16 and Bacillus subtilis Bs-20 in the pre-prepared amount of the existing NYDA culture solution and leave to ferment for 36 hours to obtain the original liquid fermentation solution, the number of bacteria reaches 10 10 More than spores / ml,

[0058] B. Prepare materials

[0059] Prepare materials according to the formula listed in Table 1. All solid additives are passed through a 100-mesh sieve, sterilized by ultraviolet light, and liquid additives should be used as new as possible to avoid contamination;

[0060] C. emulsification

[0061] Take the main drug fermentation liquid, emulsifier and antifreeze (also co-emulsifier) ​​and place them in an emulsifier to stir and emulsify for 30 minutes;

[0062] D. Add additives

[0063] Add UV protective agent, antibacterial synergist, and adhesive to the emulsion in sequence every...

Embodiment 3

[0064] The concrete preparation method of embodiment 3 is as follows:

[0065] A. Preparation of fermentation broth

[0066] Put Bacillus subtilis Bs-16 and Bacillus subtilis Bs-20 in the pre-prepared amount of the existing NYDA culture solution and leave to ferment for 48 hours to obtain the original liquid fermentation solution, the number of bacteria reaches 10 10 More than spores / ml,

[0067] B. Prepare materials

[0068] Prepare materials according to the formula listed in Table 1. All solid additives are passed through a 100-mesh sieve, sterilized by ultraviolet light, and liquid additives should be used as new as possible to avoid contamination;

[0069] C. emulsification

[0070] Take the main drug fermentation liquid, emulsifier and antifreeze (also co-emulsifier) ​​and place them in an emulsifier to stir and emulsify for 30 minutes;

[0071] D. Add additives

[0072] Add UV protective agent, antibacterial synergist, and adhesive to the emulsion in sequence every...

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Abstract

The invention discloses a multi-effect compound preparation of biocontrol bacteria for disease resistance and production increase and a preparation method thereof. The compound fermentation liquid of two biocontrol strains of Bacillus subtilis is used as the main active ingredient, and contains an emulsifier, an ultraviolet protective agent, Synergist, antifreeze, adhesive. The preparation method is that the emulsifier and the main drug are placed together in an emulsifying machine to stir and emulsify, and then add auxiliary agents successively to continue stirring and emulsifying to obtain the finished product of bacillus subtilis emulsion. The product can be quickly and evenly dispersed in water, has a wide antibacterial spectrum, has a remarkable effect of increasing production, and has a wide range of applications.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a disease-resistant and production-increasing Bacillus subtilis compound microbial pesticide and a preparation method thereof. Background technique [0002] According to FAO statistics, the annual losses caused by various diseases in the world's agriculture and forestry industry account for 12% of the total output value. In order to recover the losses, chemical pesticides play a pivotal role, but with the increase in their dosage year by year, the inherent toxicity of chemical pesticides and pesticide residues have seriously polluted the environment where human beings are lazy to live, destroyed the ecological balance, and caused a great threat to human health. It is booming and attracting more and more attention. Among them, microbial pesticides are not easy to produce drug resistance because of their control objects, do not harm natural enemies, are safe for humans and animal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01N25/04A01P3/00A01P21/00
Inventor 刘锦霞李娜李晶杜文静张建军丁品柳军玺武建荣
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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