Porphyrin derivative nano-composite preparation and its application

A technology of derivatives and composite reagents, which is applied in the preparation of porphyrin derivative nanocomposites and its application fields, can solve the problems of structural limitations, restriction of porphyrin compound physiological activity, limited selection of outer ring functional groups, etc., and achieve low toxicity , broad medical clinical application value and prospects, and the effect of easy preparation

Active Publication Date: 2014-05-28
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the structural modification of natural porphyrin can be easily modified, it is limited by the structure itself. At the same time, the choice of functional groups on the outer ring is also very limited. In addition, the physiological activity of the porphyrin compound itself is also limited.

Method used

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  • Porphyrin derivative nano-composite preparation and its application
  • Porphyrin derivative nano-composite preparation and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Interaction of composite reagents of porphyrin derivatives and nanomaterials with isolated synoviocytes

[0030] 1. Taking synoviocytes as the research object, incubate the sterile porphyrin derivative and nanomaterial composite reagent with the synoviocytes in the logarithmic growth phase for 8-24 hours (37 °C, 5 % CO 2 , RH 95%), using the MTT assay to test the cytotoxicity of different types of compound reagents interacting with normal / rheumatoid arthritis synoviocytes;

[0031] 2. Taking synoviocytes as the research object, the experimental group divided the synoviocytes in the logarithmic growth phase into 1.6×10 5 The density of cells / well was inoculated in a 6-well plate, and after 24 h of culture, the composite reagent solution of porphyrin derivatives and nanomaterials that had been sterilized and diluted with fresh sterile DMEM medium was added.

[0032] In the control group, the synoviocytes in the culture medium were divided into 1.6×10 5 Cells...

Embodiment 2

[0035] Example 2 Interaction of composite reagents of porphyrin derivatives and nanomaterials with isolated synoviocytes

[0036] 1. Taking synoviocytes as the research object, incubate the sterile porphyrin derivative and nanomaterial composite reagent with the synoviocytes in the logarithmic growth phase for 8-24 hours (37 °C, 5 % CO 2 , RH 95%), using the MTT assay to test the cytotoxicity of different types of compound reagents interacting with normal / rheumatoid arthritis synoviocytes;

[0037] 2. Taking synoviocytes as the research object, the experimental group divided the synoviocytes in the logarithmic growth phase into 1.6×10 5 The density of cells / well was inoculated in a 6-well plate, and after 24 h of culture, the composite reagent solution of porphyrin derivatives and nanomaterials that had been sterilized and diluted with fresh sterile DMEM medium was added.

[0038] In the control group, the synoviocytes in the culture medium were divided into 1.6×10 5 Cells / w...

Embodiment 3

[0041] Example 3 Interaction of composite reagents of porphyrin derivatives and nanomaterials with isolated synoviocytes

[0042] 1. Taking synoviocytes as the research object, incubate the sterile porphyrin derivative and nanomaterial composite reagent with the synoviocytes in the logarithmic growth phase for 8-24 hours (37 °C, 5 % CO 2 , RH 95%), using the MTT assay to test the cytotoxicity of different types of compound reagents interacting with normal / rheumatoid arthritis synoviocytes;

[0043] 2. Taking synoviocytes as the research object, the experimental group divided the synoviocytes in the logarithmic growth phase into 1.6×10 5 The density of cells / well was inoculated in a 6-well plate, and after 24 h of culture, the composite reagent solution of porphyrin derivatives and nanomaterials that had been sterilized and diluted with fresh sterile DMEM medium was added.

[0044] In the control group, the synoviocytes in the culture medium were divided into 1.6×10 5 Cells...

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Abstract

The invention discloses a porphyrin derivative nano-composite preparation applied in photodynamic therapy and photothermal therapy of rheumatoid arthritis and an application thereof. The invention discloses an experimental method of a composite reagent of a water-soluble porphyrin derivative and a nano-material. The composite reagent has stable fluorescence characteristic and excellent biocompatibility. According to the composite reagent, drug release detection and tracing of an inflammation area can be realized, and enrichment in the inflammation area can be achieved through porphyrin molecule absorption of the nano-material. The experimental method provided by the invention is easy to implement, and has low toxicity and high specificity. The porphyrin derivative in the obtained composite reagent can undergo violet light excitation to generate singlet oxygen. The nanometer material in the composite reagent has excellent photothermal effect under the red light excitation condition. Drug enrichment and multi-model treatment by photodynamic therapy and photothermal therapy for inflammation disease can be realized, and the porphyrin derivative nano-composite preparation has wide medical clinical application value and prospect.

Description

technical field [0001] The present invention relates to a composite reagent of porphyrin derivatives and nanomaterials used in photodynamic therapy and photothermal therapy of rheumatoid arthritis and its experimental method, in particular to the photocatalytic activity of porphyrin derivatives under purple light irradiation conditions. Kinetic therapy, photothermal therapy of nanomaterials under red light irradiation and the use of adsorption of nanomaterials to achieve drug enrichment of porphyrin derivatives in inflammatory areas, while using the stable fluorescence properties and excellent biocompatibility of composite reagents Realize the detection and tracking of drug release in the inflammatory area, so as to realize the multimodal synergistic treatment of inflammatory diseases such as rheumatoid arthritis. Background technique [0002] As we all know, rheumatoid arthritis is a chronic systemic autoimmune disease mainly characterized by joint disease. The main clinic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61P29/00A61P19/02
Inventor 王雪梅赵春秋王建玲任发
Owner SOUTHEAST UNIV
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