Single nucleotide polymorphic site rs7574865 related to liver cancer susceptibility and application thereof

A susceptibility, liver cancer technology, applied in the direction of DNA/RNA fragment, recombinant DNA technology, etc., can solve the problem of lack of relevant detection methods and detection kits, and no STAT4 gene.

Inactive Publication Date: 2014-06-04
FUDAN UNIV
View PDF1 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there has been no report on the association between the STAT4 gene involved in the present invention and liver cancer, nor ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Single nucleotide polymorphic site rs7574865 related to liver cancer susceptibility and application thereof
  • Single nucleotide polymorphic site rs7574865 related to liver cancer susceptibility and application thereof
  • Single nucleotide polymorphic site rs7574865 related to liver cancer susceptibility and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. Genotyping of SNP site rs7574865

[0038] The genotyping of SNP site rs7574865 was carried out by extracting the subjects' genomic DNA, and the results showed that the individuals whose rs7574865 base was G were the susceptible population of liver cancer.

[0039] The specific method is:

[0040] 1. Genomic DNA Extraction

[0041] All subjects were drawn 2-3ml of peripheral venous blood, and genomic DNA was extracted with QIAamp Blood Mini Kit250 (QIAGEN, Germany) kit, and stored at -20°C.

[0042] 2. SNP genotyping

[0043] PCR reactions and single-base extension primers were selected according to the sequence information of the SNP, and synthesized at Bioengineering (Shanghai) Co., Ltd. The synthesized primers were subjected to PCR reaction with the sample DNA, and the mixed reaction products were subjected to SNP genotyping on the Sequenom iPLEX instrument (Sequenom Company). The specific experimental process is as follows:

[0044] (1) Primer design:...

Embodiment 2

[0069] Example 2 Verification of genotyping of SNP site rs7574865

[0070] In the present invention, 5480 hepatitis B patients diagnosed with liver cancer are used as the research group, and 6139 hepatitis B patients without liver cancer are used as the control group. Genomic DNA was extracted from all subjects and stored at -20°C.

[0071] The results of the multi-stage case-control study showed that those whose rs7574865 base is G have a high risk of developing liver cancer, as shown in the following table:

[0072]

[0073]

[0074] It can be seen that the genotyping method for the SNP site rs7574865 of the present invention has high accuracy and reliable results.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the gene field of biological technologies, and relates to a single nucleotide polymorphic site (SNP) related to liver cancer susceptibility and application thereof. The invention firstly provides a partial sequence (shown as SEQ. ID. NO.1) of an STAT4 gene and an SNP site rs7574865 of the gene (the sequence is shown as SEQ. ID. NO.2). The invention also provides a method for detecting the SNP site, specificity nucleic acid primers and a UEP (ultra high molecular weight polyethylene) primer, wherein the sequences of the specificity nucleic acid primers are shown as SEQ.ID.NO.3 and SEQ.ID.NO.4, the sequence of the UEP primer is shown as SEQ.ID.NO.5. The invention also relates to a detection kit for the SNP site rs7574865 and application thereof. The method for genotyping of the rs7574865 site is simple and convenient and rapid, and is accurate and clear in result, and the single nucleotide polymorphic site rs7574865 provides a new way for identifying the liver cancer susceptibility and preventing and treating the liver cancer.

Description

technical field [0001] The invention belongs to the gene field of biotechnology, and specifically relates to a single nucleotide polymorphism (single nucleotide polymorphism, SNP) site related to liver cancer susceptibility and its application. Background technique [0002] Liver cancer is the seventh most common malignant tumor in the world, ranking third among cancer-related causes of death (Yang JD, Roberts LR. Hepatocel lular carcinoma: A global view. Nat Rev Gastroenterol Hepatol. 2010; 7:448-58.), It is estimated that there are 748,300 new cases and 695,900 deaths in the world every year, and more than half of the liver cancer cases are concentrated in China (Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics . CA Cancer J Clin. 2011;61:69-90.). [0003] Worldwide, chronic hepatitis B and chronic hepatitis C are the main risk factors for liver cancer. Almost 80% of liver cancer patients have a history of hepatitis B or C, and 54% of liver...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/11C12Q1/68
Inventor 余龙蒋德科徐剑锋蒋维唐丽莎
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap