Liquid way buffer type sheath fluid steady flow system

Abstract

The invention relates to a liquid way buffer type sheath fluid steady flow system. A direct-through air bag type pulse buffer comprises a cylindrical shell and a compressible telescopic air bag arranged in the shell; an output port of a peristaltic pump is communicated with an inlet of the compressible telescopic air bag by a front liquid flow conduit, and an outlet of the compressible telescopic air bag is communicated with a streaming pool by a rear liquid flow conduit; a closed space is formed between the outer wall of the compressible telescopic air bag and the inner wall of the cylindrical shell, and gas is filled into the space. The direct-through air bag type pulse buffer is arranged, so that the liquid flow pulsation is greatly reduced, and the sheath fluid can run more steadily; the length of a pipeline does not need to be prolonged, so that the liquid way buffer type sheath fluid steady flow system is small in design volume and space-saving for the low-flow peristaltic pump pulse buffering; the pressure buffer area can be adjusted by adjusting the pressure of the internal sealed gas when the buffer is manufactured, so that volume production can be realized.

Description

technical field [0001] The invention relates to a fluid path buffering type sheath fluid steady flow system applied to a flow cytometer. Background technique [0002] In the small desktop flow cytometer, in order to simplify the design and volume of the liquid circuit, a low-flow peristaltic pump is used to generate negative pressure in the liquid flow pipeline, so that the sheath fluid is pumped into the working fluid circuit. The introduction of peristaltic pumps avoids the use of positive pressure vacuum pumps, making the design and mass production of small desktop flow cytometers a reality. [0003] A peristaltic pump is like pinching a fluid-filled hose with your finger, and the fluid in the tube moves forward as the finger slides forward. The peristaltic pump is also based on the same principle except that the fingers are replaced by rollers. Fluid is pumped by alternately squeezing and releasing the pump's elastic delivery hose. Just like pinching the hose with two...

AI Technical Summary

Problems solved by technology

[0004] It is precisely because of this working principle of the peristaltic pump that it has its inherent limitations: the pump will generate a pulse flow during operation, which will cause the sheath fluid pressure to pulsate

However, the pulsation of the sheath fluid will destroy this laminar flow state, forming turbulent or turbulent flow, causing the cell suspension to deviate from the center of the liquid flow and the laser to focus on the center of the flow chamber spot, and even cause cells to overlap

Affect the detection resolution and sensitivity, directly lead to the failure of the experiment

[0007] 2) The use of multi-rotor peristaltic pumps cannot eliminate the inherent fluid path pulsation, and at the same time, it will bring about cost increases, flow rate limitations and increased wear of peristaltic pump hoses

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