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Application of salvianolic acid a in the preparation of drugs for the prevention and treatment of AIDS encephalopathy

A technology of AIDS encephalopathy and salvianolic acid, applied in the application field of drugs for the prevention and treatment of nerve damage caused by HIV-1 Tat protein

Inactive Publication Date: 2016-01-20
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are no related reports about the protection of salvia miltiorrhiza or salvianolic acid A on nerve damage caused by HIV-1 Tat protein or its application in the treatment of HAND.

Method used

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  • Application of salvianolic acid a in the preparation of drugs for the prevention and treatment of AIDS encephalopathy
  • Application of salvianolic acid a in the preparation of drugs for the prevention and treatment of AIDS encephalopathy
  • Application of salvianolic acid a in the preparation of drugs for the prevention and treatment of AIDS encephalopathy

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Embodiment 1

[0027] (1) Main reagents, cells and instruments

[0028] HIV-1Tat protein was purchased from Shenzhen Baiwansen Bio-Trade Co., Ltd. (Prospec, USA, batch number: 212PCLADEB28). Salvianolic acid A (batch number: 96574-01-5) and tanshinone IIA (batch number: 568-72-9) were purchased from Zhengzhou Fengyao Agriculture Co., Ltd. Rat adrenal pheochromoma cell line (PC12) was purchased from Shanghai Institute of Cells, Chinese Academy of Sciences. MTT was purchased from Sigma (batch number: MTBF7873V); LDH (lactate dehydrogenase) detection kit was purchased from Nanjing Jiancheng Institute of Bioengineering; Tunel detection kit was purchased from Promega, USA (batch number: 0000073091). Inactivated fetal bovine serum (batch number: 111023) was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd.; horse serum (batch number: 841843), DMEM medium (batch number: 823186) and MEM medium (batch number: 793870) were purchased from From Gibco; other reagents were of analytica...

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Abstract

The invention belongs to the technical field of pharmacy, and specifically relates to the application of salvianolic acid A in the preparation of drugs for preventing and treating AIDS encephalopathy and nerve damage caused by HIV-1 Tat protein. In the present invention, HIV-1 Tat protein is used to damage nerve cell line PC12 and nerve cells on mouse brain slices, and different concentrations of salvianolic acid A are used for protection, and MTT experiment, LDH detection and Tunel experiment are used to detect salvianolic acid A to reduce Tat The effect of protein-induced neuronal cell death; Dil gold particles were used to scatter-label dendritic spines to detect the effect of salvianolic acid A on reducing the function of Tat protein-damaged synaptic connections. Through the statistical analysis of the experimental results, it is found that salvianolic acid A has a good protective effect on nerve cell damage caused by Tat protein, and can be used to prepare drugs for preventing and treating nerve damage caused by Tat protein and AIDS encephalopathy.

Description

technical field [0001] The invention belongs to the field of pharmaceutical technology, and relates to a new application of salvianolic acid A, a water-soluble component of traditional Chinese medicine Salvia miltiorrhiza Application of protein-induced nerve injury drugs. Background technique [0002] AIDS encephalopathy, now also known as HIV-associated neurocognitive disorders (HAND, HIV-associated neurocognitive disorders), is characterized by motor, behavioral and cognitive impairments, and has recently been divided into three forms according to the severity of the disease: asymptomatic neurocognitive disorders Functional impairment (Asymptomaticneurocognitiveimpairment, ANI), mild neurocognitive dysfunction (mildneurocognitivedisorder, MND) and HIV-associated dementia (HIVassociateddementia, HAD). [0003] The detailed mechanism of HIV-1 causing nerve damage remains to be further studied, but it is certain that the neurotoxic protein of HIV-1 damages neurons directly o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/216A61P31/18A61P25/00
Inventor 刘红亮王靖凯史朝晖胡磊刘彬邓锦波徐括喜
Owner HENAN UNIVERSITY